Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, China.
Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Shaanxi Provincial People's Hospital, Xi'an, China.
PLoS One. 2024 Nov 12;19(11):e0310628. doi: 10.1371/journal.pone.0310628. eCollection 2024.
To prevent the residual sulfur dioxide in Chinese herbal medicines (CHM) caused by sulfur fumigation, which may lead to severe health issues, there is an urgent need for a rapid and quantitative detection technique. Sodium borohydride was used as a reducing agent to convert sulfur dioxide into hydrogen sulfide, which was then detected using lead acetate test strip. An accurate testing apparatus was designed, consisting of reaction bottle cap, reaction bottle, lead acetate test strip, and sulfur dioxide detector. The effect of different reaction variables on detection, including reductant quality, pH of initial media, reaction time, lead acetate concentration, and membrane type was investigated. The optimal conditions were determined by orthogonal experiments. The reaction membrane type and lead acetate concentration on the membrane were optimized to enhance detection accuracy. Standardized gray cards were fabricated and used to calibrate the detector. The detection system demonstrated an exceptional linear correlation (r2 = 0.9992), with a linear detection range of 0-750 mg·kg-1. The colored substances and sulfur-containing substances within the matrix of CHM did not affect the detection results. Therefore, the detection method exhibited superior accuracy and stability. The proposed technique proved to be swift, reliable, and provides a straightforward and convenient approach for the quantitative determination of sulfur dioxide in CHMs. The results of this work may provide insights into the development of test strips for quantitative detection.
为了防止中药材（CHM）中残留的二氧化硫（SO2）造成的严重健康问题，迫切需要一种快速定量的检测技术。本文使用硼氢化钠作为还原剂将 SO2 转化为硫化氢，然后使用醋酸铅试条进行检测。设计了一种精确的测试装置，包括反应瓶盖、反应瓶、醋酸铅试条和二氧化硫检测仪。考察了不同反应变量对检测的影响，包括还原剂质量、初始介质 pH 值、反应时间、醋酸铅浓度和膜类型。通过正交实验确定了最佳条件。优化了反应膜类型和膜上的醋酸铅浓度以提高检测精度。制作了标准化的灰度卡并用于校准检测仪。检测系统表现出优异的线性相关性（r2 = 0.9992），线性检测范围为 0-750 mg·kg-1。CHM 基质中的有色物质和含硫物质不会影响检测结果。因此，该检测方法具有较高的准确性和稳定性。该技术快速可靠，为 CHM 中 SO2 的定量测定提供了一种简单方便的方法。这项工作的结果可能为定量检测试条的开发提供思路。
