Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Department of Analytical Chemistry, Institute of Chemistry, University of Campinas - UNICAMP, 13083-970, Campinas, SP, Brazil.
Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Department of Analytical Chemistry, Institute of Chemistry, University of Campinas - UNICAMP, 13083-970, Campinas, SP, Brazil.
Talanta. 2022 Feb 1;238(Pt 1):123004. doi: 10.1016/j.talanta.2021.123004. Epub 2021 Oct 30.
Venturi easy ambient sonic spray ionization (V-EASI) is a soft ambient ionization (AI) source that has the advantages of being suitable to the analysis of samples in solution (differently from the majority of AI sources), performing self-pumping, voltage- and heat-free ionization, and requiring minimum or no sample preparation. Since this ionization technique has not been fully explored, the present study provides a proof of principle of the coupling of liquid chromatography to mass spectrometry (LC-MS) using V-EASI as the interface. In order to test the performance of the developed LC-V-EASI-MS system, a quantification method for bixin, a natural dye from annatto (Bixa Orellana L.), which is known to be sensitive to the high voltage applied for electrospray ionization mass spectrometry (ESI-MS) analysis, was validated according to FDA criteria and tested in real samples. The analytical method was successfully applied and met the validation criteria, providing a detectability 10 times better than methods already reported to the quantification of bixin and no matrix effect was observed. Therefore, this proof of principle contributes to the continuous development of AI sources that represents the last great technological advance in MS towards becoming a miniaturized technique able to analyze samples closer to their actual state.
文丘里简易环境声波喷雾电离（V-EASI）是一种软环境电离（AI）源，具有适合分析溶液中样品的优点（与大多数 AI 源不同），能够进行自抽运、无电压和无热量的电离，并且需要最少或无需样品制备。由于这种电离技术尚未得到充分探索，本研究提供了使用 V-EASI 作为接口将液相色谱与质谱（LC-MS）耦合的原理验证。为了测试开发的 LC-V-EASI-MS 系统的性能，根据 FDA 标准验证了胭脂树红（Bixa Orellana L.）天然染料胭脂红的定量方法，胭脂树红已知对用于电喷雾电离质谱（ESI-MS）分析的高压敏感，并且在实际样品中进行了测试。该分析方法成功应用并符合验证标准，与已经报道的胭脂红定量方法相比，检测灵敏度提高了 10 倍，并且没有观察到基质效应。因此，这一原理验证有助于 AI 源的不断发展，这代表了 MS 朝着成为一种能够分析更接近实际状态的样品的微型化技术的最新重大技术进步。
