Barr R D, Falbo M, Koekebakker M
Leuk Res. 1986;10(4):397-401. doi: 10.1016/0145-2126(86)90069-x.
The generation of normal human hemopoietic clones is promoted, in tissue culture, by physiological as well as pharmacological concentrations of hydrocortisone. In part this may reflect a facilitative, nutritional effect of the hormone which may be more evident in particular culture media. However, the findings, in liquid suspension cultures, of an increase in the absolute number of cells and a rise in the mitotic index in the presence of hydrocortisone, point to a real stimulation of cellular proliferation. Measurements of the number of clones early in culture, and the size of clones after longer intervals, indicate respectively that the hormone influences both recruitment of clonogenic cells into cytokinesis and amplification of established clones. The target cell in both processes may be the same, namely a morphologically recognizable entity, in the granulocyte lineage, having limited proliferative potential. Evidence in support of this interpretation includes the high cloning efficiency, small mean clonal size and brief clonal lifespan. These features suggest that members of the myeloblast-promyelocyte-myelocyte hierarchy are likely candidates, but whether the action of hydrocortisone is exerted directly on these cells, or on a more mature accessory population, remains to be determined.
在组织培养中，生理浓度及药理浓度的氢化可的松均可促进正常人造血克隆的生成。这在一定程度上可能反映了该激素的促进作用以及营养作用，而这种作用在特定培养基中可能更为明显。然而，在液体悬浮培养中发现，氢化可的松存在时细胞绝对数量增加且有丝分裂指数上升，这表明其对细胞增殖有实际的刺激作用。培养早期克隆数量的测定以及较长时间后克隆大小的测定分别表明，该激素既影响克隆形成细胞进入胞质分裂的募集过程，也影响已建立克隆的扩增。这两个过程中的靶细胞可能相同，即粒细胞系中一个形态可识别、增殖潜力有限的实体。支持这一解释的证据包括高克隆效率、小平均克隆大小和短暂的克隆寿命。这些特征表明，原粒细胞-早幼粒细胞-中幼粒细胞谱系中的成员可能是合适的候选细胞，但氢化可的松的作用是直接作用于这些细胞，还是作用于更成熟的辅助细胞群体，仍有待确定。
