Wang Kai, Li Zhongming, Ma Wenjie, Sun Yan, Liu Xianling, Qian Lijun, Hong Jian, Lu Dasheng, Zhang Jing, Xu Di
Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Department of Geriatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
J Biomed Res. 2021 Oct 10;35(6):425-435. doi: 10.7555/JBR.35.20210088.
Acute myocardial infarction (AMI) is a severe cardiovascular disease. This study aimed to identify crucial microRNAs (miRNAs) and mRNAs in AMI by establishing a miRNA-mRNA network. The microarray datasets GSE31568, GSE148153, and GSE66360 were downloaded from the Gene Expression Omnibus (GEO) database. We identified differentially expressed miRNAs (DE-miRNAs) and mRNAs (DE-mRNAs) in AMI samples compared with normal control samples. The consistently changing miRNAs in both GSE31568 and GSE148153 datasets were selected as candidate DE-miRNAs. The interactions between the candidate DE-miRNAs and DE-mRNAs were analyzed, and a miRNA-mRNA network and a protein-protein interaction network were constructed, along with functional enrichment and pathway analyses. A total of 209 DE-miRNAs in the GSE31568 dataset, 857 DE-miRNAs in the GSE148153 dataset, and 351 DE-mRNAs in the GSE66360 dataset were identified. Eighteen candidate DE-miRNAs were selected from both the GSE31568 and GSE148153 datasets. Furthermore, , , , , and were shown to have a higher degree in the miRNA-mRNA network. as well as was a hub gene in the miRNA-mRNA network and the protein-protein interaction (PPI) network, respectively. was important in both miRNA-mRNA network and PPI network. We established a miRNA-mRNA network in AMI and identified five miRNAs and three genes, which might be used as biomarkers and potential therapeutic targets for patients with AMI.
急性心肌梗死（AMI）是一种严重的心血管疾病。本研究旨在通过建立miRNA-mRNA网络来识别急性心肌梗死中的关键微小RNA（miRNA）和信使核糖核酸（mRNA）。从基因表达综合数据库（GEO）下载了微阵列数据集GSE31568、GSE148153和GSE66360。我们确定了与正常对照样本相比急性心肌梗死样本中差异表达的miRNA（DE-miRNA）和mRNA（DE-mRNA）。选择GSE31568和GSE148153数据集中均持续变化的miRNA作为候选DE-miRNA。分析了候选DE-miRNA与DE-mRNA之间的相互作用，并构建了miRNA-mRNA网络和蛋白质-蛋白质相互作用网络，同时进行了功能富集和通路分析。在GSE31568数据集中共鉴定出209个DE-miRNA，在GSE148153数据集中鉴定出857个DE-miRNA，在GSE66360数据集中鉴定出351个DE-mRNA。从GSE31568和GSE148153数据集中共选择了18个候选DE-miRNA。此外， 、 、 、 、 和 在miRNA-mRNA网络中显示出较高的度。 以及 分别是miRNA-mRNA网络和蛋白质-蛋白质相互作用（PPI）网络中的枢纽基因。 在miRNA-mRNA网络和PPI网络中都很重要。我们在急性心肌梗死中建立了miRNA-mRNA网络，并鉴定出5种miRNA和3个基因，它们可能作为急性心肌梗死患者的生物标志物和潜在治疗靶点。
