Higashimura Yasuki, Hirabayashi Misaki, Nishikawa Hitomi, Inoue Ryo, Nagai Emiko, Matsumoto Kenji, Enomoto Toshiki, Mizushima Katsura, Takagi Tomohisa, Naito Yuji
Department of Food Science, Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, Ishikawa 921-8836, Japan.
Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
J Clin Biochem Nutr. 2021 Nov;69(3):272-279. doi: 10.3164/jcbn.20-203. Epub 2021 May 28.
Consumption of yacon () is associated with beneficial effects such as prevention of metabolic diseases. Yacon root is known to contain various bioactive components including indigestible carbohydrates, but the alteration of intestinal environment after treatment with yacon has not been fully investigated. This study investigated yacon-containing diet effects on the intestinal environment in mice, including microbial composition, short-chain fatty acid levels, and mucus content. After mice were administered yacon-containing diet for 4 weeks, 16S rRNA gene sequencing analyses revealed their fecal microbiota profiles. Organic acid concentrations in cecal contents were measured using an HPLC system. Compared to the control group, yacon-containing diet-received mice had significantly higher the concentrations of succinic acid, lactic acid, acetic acid, and propionic acid. The fecal mucin content was also higher in yacon-containing diet-received mice. Results of 16S rRNA gene sequencing analyses showed that the relative abundances of 27 taxa differed significantly in yacon-containing diet-received mice. Furthermore, results show effects of yacon administration on intestinal inflammation using 2,4,6-trinitrobenzene sulfonic acid induced colitis model in mice. Increased colonic damage and myeloperoxidase activity after 2,4,6-trinitrobenzene sulfonic acid treatment were suppressed in yacon-containing diet-received mice. Results suggest that oral intake of yacon root modulates the intestinal environment, thereby inhibiting intestinal inflammation.
食用雪莲果（）与预防代谢性疾病等有益效果相关。雪莲果根已知含有多种生物活性成分，包括难消化的碳水化合物，但雪莲果处理后肠道环境的变化尚未得到充分研究。本研究调查了含雪莲果饮食对小鼠肠道环境的影响，包括微生物组成、短链脂肪酸水平和黏液含量。小鼠食用含雪莲果饮食4周后，通过16S rRNA基因测序分析揭示了它们的粪便微生物群概况。使用高效液相色谱系统测量盲肠内容物中的有机酸浓度。与对照组相比，食用含雪莲果饮食的小鼠琥珀酸、乳酸、乙酸和丙酸的浓度显著更高。食用含雪莲果饮食的小鼠粪便黏蛋白含量也更高。16S rRNA基因测序分析结果表明，食用含雪莲果饮食的小鼠中27个分类群的相对丰度有显著差异。此外，研究结果显示了雪莲果给药对使用2,4,6-三硝基苯磺酸诱导的小鼠结肠炎模型肠道炎症的影响。在食用含雪莲果饮食的小鼠中，2,4,6-三硝基苯磺酸处理后结肠损伤和髓过氧化物酶活性的增加受到抑制。结果表明，口服雪莲果根可调节肠道环境，从而抑制肠道炎症。
