Jiang Chunlei, Yue Hangyu, Yan Bing, Dong Taiji, Cui Xiangyu, Chen Peng, Wang Zengbo
College of Electrical and Information Engineering, Northeast Petroleum University, Daqing 163318, China.
School of Computer Science and Electronic Engineering, Bangor University, Dean Street, Bangor, Gwynedd, LL57 1UT, UK.
Biomed Opt Express. 2021 Oct 26;12(11):7113-7121. doi: 10.1364/BOE.437965. eCollection 2021 Nov 1.
There is a growing interest to use live cells to replace the widely used non-biological microsphere lenses. In this work, we demonstrate the use of yeast cells for such imaging purpose. Using fiber-based optical trapping technique, we trap a chain of three yeast cells and bring them to the vicinity of imaging objects. These yeast cells work as near-field magnifying lenses and simultaneously pick up the sub-diffraction information of the nanoscale objects under each cell and project them into the far-field. The experimental results demonstrated that Blu-ray disc of 100 nm feature can be clearly resolved in a parallel manner by each cell.
使用活细胞来替代广泛使用的非生物微球透镜的兴趣日益浓厚。在这项工作中，我们展示了酵母细胞用于此类成像目的的应用。利用基于光纤的光镊技术，我们捕获了一串三个酵母细胞，并将它们带到成像物体附近。这些酵母细胞充当近场放大透镜，同时获取每个细胞下方纳米级物体的亚衍射信息，并将其投射到远场。实验结果表明，每个细胞都能以并行方式清晰分辨出特征尺寸为100 nm的蓝光光盘。
