Pirone Daniele, Sirico Daniele G, Mugnano Martina, Del Giudice Danila, Kurelac Ivana, Cavina Beatrice, Memmolo Pasquale, Miccio Lisa, Ferraro Pietro
Department of Electrical Engineering and Information Technologies, University of Naples "Federico II", via Claudio 21, 80125 Napoli, Italy.
CNR-ISASI, Institute of Applied Sciences and Intelligent Systems "E. Caianiello", Via Campi Flegrei 34, 80078 Pozzuoli, Napoli, Italy.
Biomed Opt Express. 2022 Oct 4;13(11):5585-5598. doi: 10.1364/BOE.460204. eCollection 2022 Nov 1.
In recent years, intracellular LDs have been discovered to play an important role in several pathologies. Therefore, detection of LDs would provide an in-demand diagnostic tool if coupled with flow-cytometry to give significant statistical analysis and especially if the diagnosis is made in full non-invasive mode. Here we combine the experimental results of in-flow tomographic phase microscopy with a suited numerical simulation to demonstrate that intracellular LDs can be easily detected through a label-free approach based on the direct analysis of the 2D quantitative phase maps recorded by a holographic flow cytometer. In fact, we demonstrate that the presence of LDs affects the optical focusing lensing features of the embracing cell, which can be considered a biological lens. The research was conducted on white blood cells (i.e., lymphocytes and monocytes) and ovarian cancer cells. Results show that the biolens properties of cells can be a rapid biomarker that aids in boosting the diagnosis of LDs-related pathologies by means of the holographic flow-cytometry assay for fast, non-destructive, and high-throughput screening of statistically significant number of cells.
近年来，人们发现细胞内脂滴在多种病理过程中发挥着重要作用。因此，如果将脂滴检测与流式细胞术相结合以进行有效的统计分析，特别是如果能够以完全非侵入性的方式进行诊断，那么脂滴检测将提供一种急需的诊断工具。在此，我们将流动层析相显微镜的实验结果与合适的数值模拟相结合，以证明通过基于对全息流式细胞仪记录的二维定量相图进行直接分析的无标记方法，可以轻松检测细胞内脂滴。事实上，我们证明脂滴的存在会影响包围细胞的光学聚焦透镜特性，细胞可被视为一个生物透镜。该研究针对白细胞（即淋巴细胞和单核细胞）以及卵巢癌细胞展开。结果表明，细胞的生物透镜特性可以作为一种快速生物标志物，通过全息流式细胞术检测法，有助于促进对与脂滴相关病理的诊断，从而对大量细胞进行快速、无损且高通量的统计学意义显著的筛选。
