通过荧光测量实时DNA/RNA核酸酶活性
Measuring Real-time DNA/RNA Nuclease Activity through Fluorescence.
作者信息
Wyrzykowska Paulina, Rogers Sally, Chahwan Richard
机构信息
Institute of Experimental Immunology, University of Zurich, 8057 Zurich, Switzerland.
Living Systems Institute, University of Exeter, Exeter EX4 4QD, UK.
出版信息
Bio Protoc. 2021 Nov 5;11(21):e4206. doi: 10.21769/BioProtoc.4206.
DNA and RNA nucleases are wide-ranging enzymes, taking part in broad cellular processes from DNA repair to immune response control. Growing interest in the mechanisms and activities of newly discovered nucleases inspired us to share the detailed protocol of our nuclease assay ( Sheppard , 2019 ). This easy and inexpensive method can provide data that enables understanding of the molecular mechanism for novel or tested nucleases, from substrate preference and cofactors involved to catalytic rate of reaction.
DNA和RNA核酸酶是一类广泛存在的酶，参与从DNA修复到免疫反应控制等广泛的细胞过程。人们对新发现核酸酶的机制和活性越来越感兴趣，这促使我们分享核酸酶测定的详细方案（谢泼德，2019年）。这种简单且成本低廉的方法能够提供相关数据，有助于理解新型或已测试核酸酶的分子机制，包括底物偏好、涉及的辅助因子以及催化反应速率等。
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