Departamento de Patología, Hospital Ángeles Lomas, Estado de México, Mexico.
Departamento de Patología, Centro Medico ABC, Ciudad de México, Mexico.
Methods Mol Biol. 2022;2422:203-216. doi: 10.1007/978-1-0716-1948-3_14.
The conventional hematoxylin and eosin stain (H&E) is vital for the histological diagnosis but the role of immunohistochemistry (IHC) in the central nervous system is undeniable. Immunohistochemical techniques detect antigens in tissue sections by immunological and chemical reactions. This chapter reviews the preanalytic, analytic, and postanalytic phases of immunohistochemistry, as well as the principles of quality control and validation.
常规苏木精-伊红染色（H&E）对组织学诊断至关重要，但免疫组织化学（IHC）在中枢神经系统中的作用不可否认。免疫组织化学技术通过免疫学和化学反应检测组织切片中的抗原。本章回顾了免疫组织化学的分析前、分析和分析后阶段，以及质量控制和验证的原则。
