Centre for Regenerative Medicine "S. Ferrari", Department of Life Sciences, University of Modena and Reggio Emilia, 41124 Modena, Italy, Center for Genome research University of Modena and Reggio Emilia, 41124 Modena, Italy.
Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplantation, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, 41124 Modena, Italy.
Magnes Res. 2021 Aug 1;34(3):114-129. doi: 10.1684/mrh.2021.0490.
The hematopoietic U937 cells are able to differentiate into monocytes, macrophages, or osteoclasts when stimulated, respectively, with vitamin D3 (VD3), phorbol 12-myristate 13-acetate (PMA) or PMA plus VD3. We have previously demonstrated that magnesium (Mg) strongly potentiates the osteoclastic differentiation of U937 cells. In this study, we investigated whether such an effect may be ascribed to a capacity of Mg to modulate the monocyte differentiation of U937 cells and/or to an ability of Mg and VD3 to act directly and independently on the early phases of the osteoclastic differentiation. To address this issue, we subjected U937 cells to an individual and combined treatment with Mg and VD3 and then we analyzed, by flow cytometry and quantitative real-time polymerase chain reaction, the expression of a number of genes related to the early phases of the differentiation pathways under consideration. The results obtained indicated that Mg favors the monocyte differentiation of U937 cells induced by VD3 and at the same time, Mg contrasts the inhibitory effect that VD3 exerts on the osteoclastic differentiation in the absence of PMA. The crucial and articulated role played by Mg in diverse pathways of the osteoclastic differentiation of U973 cells is emphasized.
当分别受到维生素 D3 (VD3)、佛波醇 12-肉豆蔻酸 13-乙酸酯 (PMA) 或 PMA 加 VD3 的刺激时，造血 U937 细胞能够分化为单核细胞、巨噬细胞或破骨细胞。我们之前已经证明，镁 (Mg) 强烈增强 U937 细胞的破骨细胞分化。在这项研究中，我们研究了这种作用是否可以归因于 Mg 调节 U937 细胞单核细胞分化的能力，和/或 Mg 和 VD3 直接和独立作用于破骨细胞分化早期阶段的能力。为了解决这个问题，我们分别和联合用 Mg 和 VD3 处理 U937 细胞，然后通过流式细胞术和实时定量聚合酶链反应分析，研究了与所考虑的分化途径的早期阶段相关的一些基因的表达。结果表明，Mg 促进 U937 细胞由 VD3 诱导的单核细胞分化，同时，Mg 抵消了 VD3 在没有 PMA 的情况下对破骨细胞分化的抑制作用。强调了 Mg 在 U973 细胞破骨细胞分化的多种途径中发挥的关键和复杂作用。
