Keeling P A, Rocke D A, Nunn J F, Monk S J, Lumb M J, Halsey M J
Br J Anaesth. 1986 May;58(5):528-34. doi: 10.1093/bja/58.5.528.
The Sprague-Dawley rat was used to demonstrate the effect of nitrous oxide, with and without folinic pretreatment, on reproductive indices and fetal development. One of the objectives of the investigation was to test the hypothesis that at least some of the teratogenic effect of nitrous oxide is related to interference with folate metabolism. Groups of animals were exposed to 70-75% nitrous oxide on day 9 of pregnancy with or without folinic acid 0.1 mg i.p. 12 h before, and immediately before, exposure. Subsequent fetal development was compared with that of various control groups. There were no significant differences in fetal survival, but fetal weights were reduced in both groups exposed to nitrous oxide. Of the indices of skeletal maturity, the number of ossified sternebrae was reduced only in the nitrous oxide group not receiving folinic acid. The incidence of major skeletal abnormalities in the untreated nitrous oxide group was significantly increased to five times that of the control groups, whereas the incidence in the nitrous oxide group receiving folinic acid was not significantly different from control. It is concluded that pretreatment with folinic acid can at least partially reduce the teratogenic effects of nitrous oxide in the rat.
采用斯普拉格-道利大鼠来证明一氧化二氮在有或没有亚叶酸预处理的情况下对生殖指标和胎儿发育的影响。该研究的目标之一是检验以下假设：一氧化二氮的至少部分致畸作用与干扰叶酸代谢有关。在妊娠第9天，将动物分组，在暴露于一氧化二氮之前12小时及即将暴露之前，腹腔注射0.1毫克亚叶酸，一组动物暴露于70 - 75%的一氧化二氮中，另一组不注射亚叶酸。随后将胎儿发育情况与各个对照组进行比较。胎儿存活率没有显著差异，但暴露于一氧化二氮的两组胎儿体重均降低。在骨骼成熟指标中，仅未接受亚叶酸的一氧化二氮组骨化胸骨节数量减少。未处理的一氧化二氮组主要骨骼异常的发生率显著增加至对照组的五倍，而接受亚叶酸的一氧化二氮组的发生率与对照组无显著差异。得出的结论是，亚叶酸预处理至少可以部分降低一氧化二氮对大鼠的致畸作用。
