Leroy-Ladurie F, Luzerne C, Fournier M, Pariente R
Bull Eur Physiopathol Respir. 1986 Jan-Feb;22(1):49-54.
We investigated in the present study the distribution of anti-IgE binding cells in the trachea and in the thoracic lymph nodes of ovalbumin (OA)-sensitized rats. Sensitization of the airways was induced through a single intratracheal injection of the antigen, and was controlled by recording in vitro the antigen-induced contraction of the tracheal muscle: 12 of 15 (80%) OA-injected rats had a demonstrable status of tracheal sensitization. An immunohistochemical method was used for the localization of the anti-IgE binding cells in cryostat and in Carnoy fixed tissue sections. Virtually no cell was found to bind anti-IgE in the tracheas or in the thoracic lymph nodes from control animals. Conversely, we found in OA-sensitized rats that: 1) numerous IgE plasma cells appeared in the medullary cords of the lymph nodes (not in the trachea); 2) in tracheal sections, IgE concentrated in the cytoplasm of subepithelial mast cells and of nonciliated rounded epithelial cells (possibly the so-called globule leukocytes), but few if any mast cells of the connective tissue bound the anti-IgE conjugates.
在本研究中，我们调查了卵清蛋白（OA）致敏大鼠气管和胸段淋巴结中抗IgE结合细胞的分布情况。通过气管内单次注射抗原诱导气道致敏，并通过体外记录抗原诱导的气管肌肉收缩来进行监测：15只注射OA的大鼠中有12只（80%）呈现出气管致敏状态。采用免疫组织化学方法在低温恒温器切片和卡诺伊固定组织切片中定位抗IgE结合细胞。在对照动物的气管或胸段淋巴结中几乎未发现结合抗IgE的细胞。相反，我们在OA致敏大鼠中发现：1）大量IgE浆细胞出现在淋巴结的髓索中（而非气管中）；2）在气管切片中，IgE集中在上皮下单核细胞和非纤毛圆形上皮细胞（可能是所谓的球样白细胞）的细胞质中，但结缔组织中的肥大细胞极少（如果有的话）结合抗IgE结合物。
