Carswell F, Oliver J
Immunology. 1980 Jul;40(3):311-5.
PVG/c inbred rats were sensitized by intra-peritoneal injection of DNP 19-ovalbumin with or without heat-killed Bordetella pertussis as adjuvant. The use of adjuvant was associated with a greater serum IgE, greater cutaneous immediate hypersensitivity, but no increase in immediate pulmonary reactivity to aerosol challenge. The number of mast cells in the trachea of adjuvant-sensitized rats was significantly reduced when compared with both unsensitized and allergen sensitized rats but this reduction in mast cell number did not occur in the skin. The reduction in tracheal and possibly other airway mast cells may explain the failure of the adjuvant sensitized rats to show increased pulmonary reactivity despite the increased serum IgE. The B. pertussis may have acted on the mediastinal lymph nodes, which drain both the peritoneal cavity and the lungs, to produce the observed reduction in tracheal mast cells.
将PVG/c近交系大鼠通过腹腔注射19-二硝基苯基卵清蛋白（DNP 19-ovalbumin）进行致敏，注射时添加或不添加热灭活的百日咳博德特氏菌作为佐剂。使用佐剂与更高的血清IgE、更强的皮肤速发型超敏反应相关，但对气雾剂激发的即时肺反应性没有增加。与未致敏和变应原致敏的大鼠相比，佐剂致敏大鼠气管中的肥大细胞数量显著减少，但皮肤中未出现这种肥大细胞数量的减少。气管及可能其他气道肥大细胞数量的减少可能解释了尽管血清IgE增加，但佐剂致敏大鼠的肺反应性并未增加的原因。百日咳博德特氏菌可能作用于纵隔淋巴结，该淋巴结引流腹腔和肺部，从而导致观察到的气管肥大细胞减少。
