Razzeca K J, Pillemer E, Weissman I L, Rouse R V
Eur J Immunol. 1986 Apr;16(4):393-9. doi: 10.1002/eji.1830160414.
The phosphorylcholine idiotype (Id)/anti-Id system has been used to study the role of antigen-specific cells in antigen-induced microenvironmental changes. Anti-Id staining of lymph nodes following PC immunization shows the presence of Id on follicular dendritic cells at 12 h and in plasma cells beginning at day 3. Germinal centers began to form at day 3, peaking in size and number at days 8-10. Scattered Id-positive small lymphocytes are present in germinal centers but with rare exceptions over 98% of germinal center cells are Id-negative. Idiotype-positive small lymphocytes are depleted from primary follicles adjacent to germinal centers but not from distant, unstimulated nodes. These results extend previous studies showing architectural alterations in lymph nodes following antigenic stimulation and demonstrate antigen-specific cells are a prominent component of these antigen-induced microenvironmental changes.
磷酰胆碱独特型（Id）/抗独特型系统已被用于研究抗原特异性细胞在抗原诱导的微环境变化中的作用。PC免疫后淋巴结的抗Id染色显示，12小时时滤泡树突状细胞上存在Id，从第3天开始在浆细胞中出现。生发中心在第3天开始形成，在第8 - 10天大小和数量达到峰值。生发中心存在散在的Id阳性小淋巴细胞，但除极少数例外，超过98%的生发中心细胞是Id阴性的。独特型阳性小淋巴细胞从与生发中心相邻的初级滤泡中耗尽，但在远处未受刺激的淋巴结中则未耗尽。这些结果扩展了先前的研究，显示了抗原刺激后淋巴结的结构改变，并证明抗原特异性细胞是这些抗原诱导的微环境变化的一个重要组成部分。
