Nassar Osama, Desouky Said E, El-Sherbiny Gamal M, Abu-Elghait Mohammed
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 11884, Cairo, Egypt.
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 11884, Cairo, Egypt.
Microb Pathog. 2022 Jan;162:105339. doi: 10.1016/j.micpath.2021.105339. Epub 2021 Dec 1.
Pseudomonas aeruginosa is a ubiquitous pathogen capable of infecting virtually all tissues and its one of the standout amongst the most hazardous microorganisms of high morbidity and mortality rates especially in debilitated patients with few successful antibiotic choices available. This pathogen regulating most virulence traits by that so-called quorum sensing (QS), a cell to cell communication system. the present study was intended to phenotypically evaluate the activity of specific virulence traits (including swarming and swimming motility, protease, pyocyanin, and biofilm production) in Pseudomonas aeruginosa clinical isolates and assess the statistical correlation between these traits and antibiotic resistance. One hundred and thirteen bacterial isolates were obtained from different clinical samples and identified as P. aeruginosa, among them, 73.4% have the ability to forming biofilm with different degrees; 59.2% were able to produce pyocyanin pigment while all isolates having the ability to make swarming and swimming motility and able to produce protease enzyme with different degrees. The isolates that produce the higher levels of the virulence traits were identified by both biochemical using Vitek2 automated system and genetically via 16s rRNA gene analysis. The statistical analysis results indicate that a positive significant correlation was found between biofilm formation and other studied virulence traits except for protease (r = 0.584: 0.324, P < 0.05) while a non-significant correlation was found between biofilm formation and protease activity (r = 0.105, P ˃ 0.05). Swimming and swarming motility have a positive significant correlation with other studied virulence traits (r = 0.613: 0.297, P < 0.05) except for protease. Pyocyanin pigment production have a positive significant correlation with other studied virulence traits (r = 0.33: 0.297, P < 0.05) except for protease. on the other hand, negative significant correlations were found between biofilm formation, swimming; and swarming motility, Pyocyanin pigment production, and the susceptibility of antibiotics (r = -0.512: -0.281, P < 0.05). Detection of such correlations in P. aeruginosa is useful for study the behavior of this pathogen and may be provide a new target for the treatment of MDR infections.
铜绿假单胞菌是一种无处不在的病原体，几乎能够感染所有组织，在发病率和死亡率极高的最危险微生物中，它是其中之一，尤其是在几乎没有有效抗生素选择的虚弱患者中。这种病原体通过所谓的群体感应（QS）来调节大多数毒力特性，QS是一种细胞间通讯系统。本研究旨在从表型上评估铜绿假单胞菌临床分离株中特定毒力特性（包括群集和游动运动性、蛋白酶、绿脓菌素和生物膜形成）的活性，并评估这些特性与抗生素耐药性之间的统计相关性。从不同临床样本中获得了113株细菌分离株，并鉴定为铜绿假单胞菌，其中73.4%有能力形成不同程度的生物膜；59.2%能够产生绿脓菌素色素，而所有分离株都有能力进行不同程度的群集和游动运动，并能够产生蛋白酶。通过使用Vitek2自动化系统进行生化鉴定以及通过16s rRNA基因分析进行基因鉴定，确定了产生较高水平毒力特性的分离株。统计分析结果表明，除蛋白酶外，生物膜形成与其他研究的毒力特性之间存在显著正相关（r = 0.584: 0.324，P < 0.05），而生物膜形成与蛋白酶活性之间存在非显著相关性（r = 0.105，P ˃ 0.05）。除蛋白酶外，游动和群集运动性与其他研究的毒力特性之间存在显著正相关（r = 0.613: 0.297，P < 0.05）。绿脓菌素色素产生与其他研究的毒力特性之间存在显著正相关（r = 0.33: 0.297，P < 0.05），但与蛋白酶无关。另一方面，生物膜形成、游动和群集运动性、绿脓菌素色素产生与抗生素敏感性之间存在显著负相关（r = -0.512: -0.281，P < 0.05）。检测铜绿假单胞菌中的这种相关性有助于研究该病原体的行为，并可能为治疗多重耐药感染提供新的靶点。
