Porto Conte Ricerche Srl, Science and Technology Park of Sardinia, Alghero, Italy.
Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
NPJ Biofilms Microbiomes. 2021 Dec 3;7(1):85. doi: 10.1038/s41522-021-00256-x.
Diet is a key factor influencing gut microbiota (GM) composition and functions, which in turn affect host health. Among dietary regimens, time-restricted (TR) feeding has been associated to numerous health benefits. The impact of TR feeding on the GM composition has been mostly explored by means of metagenomic sequencing. To date, however, little is known about the modulation of GM functions by this dietary regimen. Here, we analyzed the effects of TR feeding on GM functions by evaluating protein expression changes in a rat model through a metaproteomic approach. We observed that TR feeding has a relevant impact on GM functions, specifically leading to an increased abundance of several enzymes involved in carbohydrate and protein metabolism and expressed by Lactobacillus spp. and Akkermansia muciniphila. Taken together, these results contribute to deepening our knowledge about the key relationship between diet, GM, and health.
饮食是影响肠道微生物群（GM）组成和功能的关键因素，而 GM 又反过来影响宿主健康。在饮食方案中，限时喂养（TR）与许多健康益处有关。TR 喂养对 GM 组成的影响主要通过宏基因组测序来探索。然而，迄今为止，人们对这种饮食方案对 GM 功能的调节知之甚少。在这里，我们通过代谢组学方法在大鼠模型中评估蛋白质表达变化来分析 TR 喂养对 GM 功能的影响。我们观察到，TR 喂养对 GM 功能有显著影响，特别是导致参与碳水化合物和蛋白质代谢的几种酶的丰度增加，这些酶由 Lactobacillus spp. 和 Akkermansia muciniphila 表达。总之，这些结果有助于加深我们对饮食、GM 和健康之间关键关系的认识。
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