使用针对染色体区域14q11的DNA探针检测人抗原特异性T淋巴细胞克隆中的T细胞受体α链基因座重排。

Detection of T cell receptor alpha chain locus rearrangements in human antigen-specific T lymphocyte clones using a DNA probe specific for chromosome region 14q11.

作者信息

Mathieu-Mahul D, Chen L K, Larsen C J, Bensussan A

出版信息

J Exp Med. 1986 May 1;163(5):1308-13. doi: 10.1084/jem.163.5.1308.

DOI:10.1084/jem.163.5.1308

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2188096/

Abstract

Human cloned antigen-specific T lymphocytes were used to characterize DNA rearrangements using a cDNA probe from the T cell receptor (TCR)-alpha chain gene. Rearranged patterns were detected in some T cell clones, confirming that normal mature T cells are rearranged in TCR-alpha locus. Similarly, rearranged DNA patterns were found in T cell clones from the same panel, using a DNA probe (clone K-40, [1]) isolated from chromosome 14 (14q11), where the TCR-alpha locus has been mapped. These results suggest that this genomic DNA clone is located within the TCR-alpha chain locus.

摘要

利用人克隆的抗原特异性T淋巴细胞，通过来自T细胞受体（TCR）α链基因的cDNA探针来鉴定DNA重排。在一些T细胞克隆中检测到重排模式，证实正常成熟T细胞在TCR-α基因座中发生了重排。同样，使用从14号染色体（14q11）分离的DNA探针（克隆K-40，[1]），在同一组的T细胞克隆中发现了重排的DNA模式，TCR-α基因座已定位在该染色体上。这些结果表明，该基因组DNA克隆位于TCR-α链基因座内。

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Detection of T cell receptor alpha chain locus rearrangements in human antigen-specific T lymphocyte clones using a DNA probe specific for chromosome region 14q11.使用针对染色体区域14q11的DNA探针检测人抗原特异性T淋巴细胞克隆中的T细胞受体α链基因座重排。

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本文引用的文献

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Histocompatibility-linked immune response genes.组织相容性连锁免疫反应基因

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Science. 1985 Mar 1;227(4690):1044-7. doi: 10.1126/science.3919442.

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