Mathieu-Mahul D, Chen L K, Larsen C J, Bensussan A
J Exp Med. 1986 May 1;163(5):1308-13. doi: 10.1084/jem.163.5.1308.
Human cloned antigen-specific T lymphocytes were used to characterize DNA rearrangements using a cDNA probe from the T cell receptor (TCR)-alpha chain gene. Rearranged patterns were detected in some T cell clones, confirming that normal mature T cells are rearranged in TCR-alpha locus. Similarly, rearranged DNA patterns were found in T cell clones from the same panel, using a DNA probe (clone K-40, [1]) isolated from chromosome 14 (14q11), where the TCR-alpha locus has been mapped. These results suggest that this genomic DNA clone is located within the TCR-alpha chain locus.
利用人克隆的抗原特异性T淋巴细胞,通过来自T细胞受体(TCR)α链基因的cDNA探针来鉴定DNA重排。在一些T细胞克隆中检测到重排模式,证实正常成熟T细胞在TCR-α基因座中发生了重排。同样,使用从14号染色体(14q11)分离的DNA探针(克隆K-40,[1]),在同一组的T细胞克隆中发现了重排的DNA模式,TCR-α基因座已定位在该染色体上。这些结果表明,该基因组DNA克隆位于TCR-α链基因座内。