Bensussan A, Acuto O, Hussey R E, Milanese C, Reinherz E L
Nature. 1984;311(5986):565-7. doi: 10.1038/311565a0.
T3-associated disulphide linked heterodimers (Tin) comprised of clonally unique alpha-chains of molecular weight (MW) 49,000-54,000 and beta chains of MW 43,000 have been identified as the antigen receptors on human cytotoxic effector and inducer T-lymphocytes. Crosslinking of Ti molecules by either the appropriate nominal antigen/MHC specificity or anti-clonotypic monoclonal antibody results in clonal expansion of such cells via induction of IL-2 receptor expression, endogenous IL-2 release and IL-2-IL-2 receptor interaction. To determine whether analogous antigen receptor molecules and autocrine growth mechanisms are utilized by suppressor T-cells, we produced an anti-clonotypic monoclonal antibody against a non-cytotoxic T8+ suppressor T-cell, T8AC6, which defines a T3-associated disulphide-linked heterodimer of similar molecular weight to the above clonotypes. We find that Te-Ti triggering of suppressor clones (T8AC6, T8AC7 or T8RW) does not result in IL-2 production or T-cell proliferation and in contrast to inducer clones, also leads to a transient IL-2 unresponsive state. We suggest that such T3-Ti receptor mediated autoregulation of suppressor T-cell growth is necessary in the facilitation of initial inducer T-cell activation following antigenic perturbation.
由分子量（MW）为49,000 - 54,000的克隆独特α链和MW为43,000的β链组成的T3相关二硫键连接的异二聚体（Tin）已被鉴定为人类细胞毒性效应细胞和诱导性T淋巴细胞上的抗原受体。通过适当的名义抗原/MHC特异性或抗克隆型单克隆抗体使Ti分子交联，会通过诱导IL-2受体表达、内源性IL-2释放和IL-2 - IL-2受体相互作用导致此类细胞的克隆扩增。为了确定抑制性T细胞是否利用类似的抗原受体分子和自分泌生长机制，我们制备了一种针对非细胞毒性T8 +抑制性T细胞T8AC6的抗克隆型单克隆抗体，该抗体界定了一种与上述克隆型分子量相似的T3相关二硫键连接的异二聚体。我们发现，抑制性克隆（T8AC6、T8AC7或T8RW）的Te - Ti触发不会导致IL-2产生或T细胞增殖，并且与诱导性克隆相反，还会导致短暂的IL-2无反应状态。我们认为，这种T3 - Ti受体介导的抑制性T细胞生长的自动调节对于抗原扰动后促进初始诱导性T细胞活化是必要的。
