College of Health and Natural Sciences, Franklin Pierce University, Rindge, New Hampshire, USA.
Biochem Mol Biol Educ. 2022 Jan;50(1):142-148. doi: 10.1002/bmb.21593. Epub 2021 Dec 4.
We present a course-embedded undergraduate research module that involves real-time polymerase chain reaction testing for the presence of SARS-CoV-2 in environmental samples. A positive control RNA was constructed and two RNA extraction methods were compared and a range of primers were available to compare. Using a combination of published protocols, we assembled a successful project that illustrated a topical exercise similar to real-world assay development. The exercise is aimed at upper-level undergraduates and requires 3 weeks of laboratory periods. The students were able to design and test experimental protocols, while learning about RNA detection. This project could be utilized in upper-level classes including molecular biology, biochemistry, biotechnology, or for independent research projects.
我们提出了一个课程嵌入式本科研究模块，该模块涉及实时聚合酶链反应测试，以检测环境样本中 SARS-CoV-2 的存在。构建了阳性对照 RNA，并比较了两种 RNA 提取方法，并提供了一系列引物进行比较。使用组合的已发表方案，我们成功地完成了一个项目，该项目说明了与真实世界分析开发类似的专题练习。该练习针对高年级本科生，需要 3 周的实验室时间。学生可以设计和测试实验方案，同时了解 RNA 检测。该项目可用于分子生物学、生物化学、生物技术等高级课程，或用于独立的研究项目。
