Department of Plant Systems Biology, VIB, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
Structural Biology Research Center, VIB, 1050 Brussel, Belgium; Research Unit of Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussel, Belgium.
Trends Biotechnol. 2014 May;32(5):263-70. doi: 10.1016/j.tibtech.2014.03.001. Epub 2014 Apr 1.
Since the serendipitous discovery 20 years ago of bona fide camelid heavy-chain antibodies, their single-domain antigen-binding fragments, known as VHHs or nanobodies, have received a progressively growing interest. As a result of the beneficial properties of these stable recombinant entities, they are currently highly valued proteins for multiple applications, including fundamental research, diagnostics, and therapeutics. Today, with the original patents expiring, even more academic and industrial groups are expected to explore innovative VHH applications. Here, we provide a thorough overview of novel implementations of VHHs as research and diagnostic tools, and of the recently evaluated production platforms for several VHHs and VHH-derived antibody formats.
自 20 年前偶然发现真正的骆驼重链抗体以来，其单域抗原结合片段，即 VHH 或纳米抗体，受到了越来越多的关注。由于这些稳定的重组实体具有有益的特性，它们目前是多种应用（包括基础研究、诊断和治疗）中非常有价值的蛋白质。如今，随着原始专利的到期，预计甚至会有更多的学术和工业团体探索 VHH 的创新应用。在这里，我们全面概述了 VHH 作为研究和诊断工具的新应用，以及最近评估的几种 VHH 和 VHH 衍生抗体形式的生产平台。
