Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August University of Göttingen, 37077, Göttingen, Germany; Department of Earth and Environmental Sciences, Macquarie University, Sydney, NSW, 2109, Australia.
Geobiology, Geoscience Centre, Georg-August University of Göttingen, 37077, Göttingen, Germany.
Chemosphere. 2022 Feb;289:133143. doi: 10.1016/j.chemosphere.2021.133143. Epub 2021 Dec 2.
The slow rate of natural attenuation of organic pollutants, together with unwanted environmental impacts of traditional remediation strategies, has necessitated the exploration of plant-microbe systems for enhanced bioremediation applications. The identification of microorganisms capable of promoting rhizoremediation through both plant growth-promoting and hydrocarbon-degrading processes is crucial to the success and adoption of plant-based remediation techniques. In this study, through successive enrichments of soil samples from a historic oil-contaminated site in Wietze, Germany, we isolated a plant growth-promoting and hydrocarbon-degrading bacterial consortium dominated by Alphaproteobacteria. In microcosm experiments involving Medicago sativa L. and the isolated bacterial consortium, we examined the ability of the consortium to enhance rhizoremediation of petroleum hydrocarbons. The inoculation of M. sativa with the consortium resulted in 66% increase in plant biomass, and achieved a 91% reduction in diesel fuel hydrocarbon concentrations in the soil within 60 days. Metagenome analysis led to the identification of genes and taxa putatively involved in these processes. The majority of the coding DNA sequences associated with plant growth promotion and hydrocarbon degradation in this study were affiliated to Acidocella aminolytica and Acidobacterium capsulatum indicating their potential for biotechnological applications in the rhizoremediation of sites contaminated by petroleum-derived organic pollutants.
有机污染物自然衰减速度缓慢，加上传统修复策略带来的不良环境影响，促使人们探索植物-微生物系统以增强生物修复应用。确定能够通过促进植物生长和降解碳氢化合物来促进根际修复的微生物，对于成功采用基于植物的修复技术至关重要。在这项研究中，我们通过对德国维策历史上石油污染地点的土壤样本进行连续富集，分离出了一个以α变形菌为主的具有植物促生和烃类降解能力的细菌共生体。在涉及紫花苜蓿和分离出的细菌共生体的微宇宙实验中，我们研究了共生体增强石油烃类根际修复的能力。将共生体接种到紫花苜蓿中，可使植物生物量增加 66%，并在 60 天内使土壤中柴油燃料烃浓度降低 91%。宏基因组分析导致了鉴定出可能参与这些过程的基因和分类群。本研究中与植物生长促进和烃类降解相关的大多数编码 DNA 序列与 Acidocella aminolytica 和 Acidobacterium capsulatum 有关，这表明它们在受石油源有机污染物污染的场地的根际修复中有潜在的生物技术应用前景。
