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人粒细胞巨噬细胞集落刺激因子的基因组克隆、特性鉴定及多谱系生长促进活性

Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor.

作者信息

Kaushansky K, O'Hara P J, Berkner K, Segal G M, Hagen F S, Adamson J W

出版信息

Proc Natl Acad Sci U S A. 1986 May;83(10):3101-5. doi: 10.1073/pnas.83.10.3101.

DOI:10.1073/pnas.83.10.3101
PMID:3486413
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC323460/
Abstract

Through the use of long single-sequence oligonucleotide probes, the complete gene for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) has been cloned from a human genomic library. The gene is 2.5 kilobases in length, contains three introns, and is present as a single copy in the human genome. When subcloned into the mammalian expression vector pD3, the gene directs the synthesis of authentic hGM-CSF. In addition to its stimulation of in vitro granulopoiesis and monopoiesis, recombinant hGM-CSF stimulates in vitro erythropoiesis and megakaryopoiesis.

摘要

通过使用长单序列寡核苷酸探针，已从人类基因组文库中克隆出人类粒细胞-巨噬细胞集落刺激因子（hGM-CSF）的完整基因。该基因长度为2.5千碱基，包含三个内含子，在人类基因组中以单拷贝形式存在。当亚克隆到哺乳动物表达载体pD3中时，该基因指导合成天然的hGM-CSF。重组hGM-CSF除了刺激体外粒细胞生成和单核细胞生成外，还刺激体外红细胞生成和巨核细胞生成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d43/323460/32f169cf7c13/pnas00314-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d43/323460/32f169cf7c13/pnas00314-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d43/323460/32f169cf7c13/pnas00314-0063-a.jpg

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本文引用的文献

1
Codon catalog usage is a genome strategy modulated for gene expressivity.密码子编目使用是一种为基因表达性而调节的基因组策略。
Nucleic Acids Res. 1981 Jan 10;9(1):r43-74. doi: 10.1093/nar/9.1.213-b.
2
A catalogue of splice junction sequences.剪接连接序列目录。
Nucleic Acids Res. 1982 Jan 22;10(2):459-72. doi: 10.1093/nar/10.2.459.
3
The nature and action of granulocyte-macrophage colony stimulating factors.粒细胞-巨噬细胞集落刺激因子的性质与作用
牛αs1-酪蛋白基因序列指导人粒细胞-巨噬细胞集落刺激因子在转基因小鼠乳汁中高水平表达。
Transgenic Res. 1997 Jan;6(1):75-84. doi: 10.1023/a:1018461201385.
4
T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines.T细胞白细胞介素-17诱导基质细胞产生促炎和造血细胞因子。
J Exp Med. 1996 Jun 1;183(6):2593-603. doi: 10.1084/jem.183.6.2593.
5
Regulation of proliferation and cytokine expression of bone marrow fibroblasts: role of c-myb.骨髓成纤维细胞增殖和细胞因子表达的调控：c-myb的作用
J Exp Med. 1993 Sep 1;178(3):997-1005. doi: 10.1084/jem.178.3.997.
6
Thrombopoietin expands erythroid progenitors, increases red cell production, and enhances erythroid recovery after myelosuppressive therapy.血小板生成素可扩增红系祖细胞，增加红细胞生成，并增强骨髓抑制治疗后的红系恢复。
J Clin Invest. 1995 Sep;96(3):1683-7. doi: 10.1172/JCI118210.
7
Granulocyte-macrophage colony-stimulating factor mimicry and receptor interactions.粒细胞-巨噬细胞集落刺激因子模拟与受体相互作用
Immunol Res. 1994;13(2-3):96-109. doi: 10.1007/BF02918271.
8
Thrombopoietin, the Mp1 ligand, is essential for full megakaryocyte development.血小板生成素，即Mp1配体，对于巨核细胞的完全发育至关重要。
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3234-8. doi: 10.1073/pnas.92.8.3234.
9
Growth of human hemopoietic colonies in response to recombinant gibbon interleukin 3: comparison with human recombinant granulocyte and granulocyte-macrophage colony-stimulating factor.重组长臂猿白细胞介素3刺激下人类造血集落的生长：与重组人粒细胞及粒细胞-巨噬细胞集落刺激因子的比较
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6765-9. doi: 10.1073/pnas.84.19.6765.
10
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Proc Natl Acad Sci U S A. 1986 Oct;83(19):7467-71. doi: 10.1073/pnas.83.19.7467.
Blood. 1980 Dec;56(6):947-58.
4
Structure-function studies of a colony stimulating factor (CSF-1).集落刺激因子（CSF-1）的结构-功能研究
J Biol Chem. 1982 Nov 25;257(22):13679-84.
5
Enhancement of human blood eosinophil cytotoxicity by semi-purified eosinophil colony-stimulating factor(s).半纯化的嗜酸性粒细胞集落刺激因子增强人血嗜酸性粒细胞的细胞毒性
J Exp Med. 1982 Jul 1;156(1):90-103. doi: 10.1084/jem.156.1.90.
6
Interaction of lactoferrin, monocytes, and T lymphocyte subsets in the regulation of steady-state granulopoiesis in vitro.乳铁蛋白、单核细胞和T淋巴细胞亚群在体外稳态粒细胞生成调节中的相互作用。
J Clin Invest. 1981 Jul;68(1):56-63. doi: 10.1172/jci110254.
7
Direct stimulation by purified GM-CSF of the proliferation of multipotential and erythroid precursor cells.纯化的粒细胞-巨噬细胞集落刺激因子对多能和红系祖细胞增殖的直接刺激作用。
Blood. 1980 Jan;55(1):138-47.
8
Polycythemia vera: studies of hemopoiesis in continuous long-term culture of human marrow.真性红细胞增多症：人骨髓长期连续培养中的造血研究
J Cell Physiol Suppl. 1982;1:79-85. doi: 10.1002/jcp.1041130413.
9
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.真核生物mRNA翻译起始位点上游序列的汇编与分析。
Nucleic Acids Res. 1984 Jan 25;12(2):857-72. doi: 10.1093/nar/12.2.857.
10
Human megakaryocytic progenitors (CFU-M) assayed in methylcellulose: physical characteristics and requirements for growth.在甲基纤维素中检测的人巨核细胞祖细胞（CFU-M）：物理特性和生长需求。
J Cell Physiol. 1984 Jan;118(1):87-96. doi: 10.1002/jcp.1041180115.