Centre for Energy and Environment, Malaviya National Institute of Technology Jaipur, Jaipur, 302017, Rajasthan, India.
BioX Centre, School of Basic Sciences, Indian Institute of Technology Mandi, Kamand, 175075, Himachal Pradesh, India.
Metab Eng. 2022 Jan;69:323-337. doi: 10.1016/j.ymben.2021.11.014. Epub 2021 Dec 2.
Anaerobic digestion is a promising method for energy recovery through conversion of organic waste to biogas and other industrial valuables. However, to tap the full potential of anaerobic digestion, deciphering the microbial metabolic pathway activities and their underlying bioenergetics is required. In addition, the behavior of organisms in consortia along with the analytical abilities to kinetically measure their metabolic interactions will allow rational optimization of the process. This review aims to explore the metabolic bottlenecks of the microbial communities adopting latest advances of profiling and C tracer-based analysis using state of the art analytical platforms (GC, GC-MS, LC-MS, NMR). The review summarizes the phases of anaerobic digestion, the role of microbial communities, key process parameters of significance, syntrophic microbial interactions and the bottlenecks that are critical for optimal bioenergetics and enhanced production of valuables. Considerations into the designing of efficient synthetic microbial communities as well as the latest advances in capturing their metabolic cross talk will be highlighted. The review further explores how the presence of additives and inhibiting factors affect the metabolic pathways. The critical insight into the reaction mechanism covered in this review may be helpful to optimize and upgrade the anaerobic digestion system.
厌氧消化是一种有前途的能源回收方法，通过将有机废物转化为沼气和其他工业有价值的物质来实现。然而，要充分挖掘厌氧消化的潜力，需要破译微生物代谢途径的活动及其潜在的生物能量学。此外，了解生物群落中生物体的行为以及对其代谢相互作用进行动力学测量的分析能力将允许对该过程进行合理优化。本综述旨在探讨采用最新的基于剖面分析和 C 示踪剂的分析方法（GC、GC-MS、LC-MS、NMR）的微生物群落的代谢瓶颈。本综述总结了厌氧消化的各个阶段、微生物群落的作用、重要的关键工艺参数、协同微生物相互作用以及对最佳生物能量学和有价值产物的增强生产至关重要的代谢瓶颈。还将重点介绍高效合成微生物群落的设计考虑因素以及捕捉其代谢交叉对话的最新进展。本综述进一步探讨了添加剂和抑制因素的存在如何影响代谢途径。本综述涵盖的反应机制的关键见解可能有助于优化和升级厌氧消化系统。
