School of Aquatic and Fishery Sciences, University of Washington, Box 355020, Seattle, WA, 98195, USA.
School of Aquatic and Fishery Sciences, University of Washington, Box 355020, Seattle, WA, 98195, USA; US Geological Survey, Washington Cooperative Fish and Wildlife Research Unit, University of Washington, Box 355020, Seattle, WA, 98195, USA.
Mar Environ Res. 2022 Jan;173:105540. doi: 10.1016/j.marenvres.2021.105540. Epub 2021 Nov 29.
We examined the risk of withering syndrome (WS) rickettsia-like organism (WS-RLO) infection in sentinel red abalone (Haliotis rufescens) deployed in modules at two Southern California field sites, one adjacent to an abalone farm and one adjacent to wild abalones. WS-RLO DNA was detected in seawater near modules at the wild abalone site but not near the farm (WS-RLO DNA was detected in the farm effluent). More WS-RLO DNA was detected in tissue from abalone near the farm relative to those near wild abalones (p < 0.05). However, infection prevalence and intensity based on histology were low and similar between sites (p > 0.05) and were independent of WS-RLO DNA loads in abalone tissue and seawater. More stippled (ST)-RLO than WS-RLO were observed with more ST-RLO infections near wild abalone than near the abalone farm (p < 0.05). We demonstrate the utility of caged sentinel abalone to better understand pathogen transmission patterns in the field.
我们研究了在加利福尼亚南部的两个野外站点（一个紧邻鲍鱼养殖场，另一个紧邻野生鲍鱼）的模块中部署的哨兵红鲍（Haliotis rufescens）感染枯萎综合征（WS）立克次体样生物（WS-RLO）的风险。在野生鲍鱼站点的模块附近的海水中检测到 WS-RLO DNA，但在养殖场附近未检测到（在养殖场废水中检测到 WS-RLO DNA）。与靠近野生鲍鱼的鲍鱼相比，靠近养殖场的鲍鱼组织中检测到更多的 WS-RLO DNA（p<0.05）。然而，基于组织学的感染率和感染强度在两个站点之间较低且相似（p>0.05），并且与鲍鱼组织和海水中的 WS-RLO DNA 载量无关。与靠近养殖场的鲍鱼相比，靠近野生鲍鱼的鲍鱼中观察到更多的点状（ST）-RLO 感染（p<0.05）。我们证明了笼养哨兵鲍鱼在更好地理解野外病原体传播模式方面的实用性。
