School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.
College of Life Science, Hunan Normal University, Changsha, 410081, China.
Biochem Biophys Res Commun. 2022 Jan 8;587:36-41. doi: 10.1016/j.bbrc.2021.11.079. Epub 2021 Nov 24.
LncRNAs are widely involved in various biological processes of plants. Recent evidences indicated that lncRNAs could act as competing endogenous RNAs (ceRNAs) to adsorb complementary miRNAs in a type of target mimicry, thereby indirectly regulating the target genes of miRNAs. In this study, a lncRNA, lncRNA08489 was identified to be the ceRNA of miR482e-3p in tomato plants. The expression patterns of lncRNA08489 and miR482e-3p showed opposite trends after tomato plants infected with Phytophthora infestans. In tomato leaves overexpressing lncRNA08489 (OE08489), the expression level of miR482e-3p decreased and its target gene, NBS-LRR increased. After infection with P. infestans, the resistance of OE08489 plants was stronger than that of the wild type, and the reactive oxygen species (ROS) scavenging ability of OE08489 plants was significantly improved. Taken together, these results indicated that lncRNA08489 acted as a ceRNA to decoy miR482e-3p and regulate the expression of NBS-LRR to enhance tomato resistance through ROS-scavenging system.
LncRNAs 广泛参与植物的各种生物过程。最近的证据表明，lncRNAs 可以作为竞争性内源 RNA（ceRNA），通过一种靶标模拟的方式吸附互补的 miRNA，从而间接调节 miRNA 的靶基因。在本研究中，鉴定出番茄植物中的 lncRNA08489 是 miR482e-3p 的 ceRNA。番茄植物感染疫霉后，lncRNA08489 和 miR482e-3p 的表达模式呈相反趋势。在过量表达 lncRNA08489（OE08489）的番茄叶片中，miR482e-3p 的表达水平下降，其靶基因 NBS-LRR 增加。感染 P. infestans 后，OE08489 植株的抗性强于野生型，OE08489 植株的活性氧（ROS）清除能力显著提高。综上所述，这些结果表明，lncRNA08489 作为 ceRNA 与 miR482e-3p 结合，通过 ROS 清除系统调节 NBS-LRR 的表达，从而增强番茄的抗性。
