Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
J Environ Manage. 2022 Feb 15;304:114189. doi: 10.1016/j.jenvman.2021.114189. Epub 2021 Dec 1.
Colonization by sulfate-reducing bacteria (SRB) in environments associated with oil is mainly dependent on the availability of sulfate and carbon sources. The formation of biofilms by SRB increases the corrosion of pipelines and oil storage tanks, representing great occupational and operational risks and respective economic losses for the oil industry. The aim of this study was to evaluate the influence of the addition of acetate, butyrate, lactate, propionate and oil on the structure of biofilm formed in carbon steel coupons, as well as on the diversity of total bacteria and SRB in the planktonic and sessile communities from petroleum produced water. The biofilm morphology, chemical composition, average roughness and the microbial diversity was analyzed. In all carbon sources, formation of dense biofilm without morphological and/or microbial density differences was detected, with the most of cells observed in the form of individual rods. The diversity and richness indices of bacterial species in the planktonic community was greater than in the biofilm. Geotoga was the most abundant genus, and more than 85% of SRB species were common to all treatments. The functional predicted profile shown that the observed genres in planktonic communities were related to the reduction of sulfate, sulfite, elementary sulfur and other sulfur compounds, but the abundance varied between treatments. For the biofilm, the functions predicted profile for the oil treatment was the one that most varied in relation to the control, while for the planktonic community, the addition of all carbon sources interfered in the predicted functional profile. Thus, although it does not cause changes in the structure and morphology biofilm, the supplementation of produced water with different carbon sources is associated with changes in the SRB taxonomic composition and functional profiles of the biofilm and the planktonic bacterial communities.
硫酸盐还原菌（SRB）在与石油相关的环境中的定殖主要依赖于硫酸盐和碳源的可用性。SRB 形成生物膜会增加管道和油罐的腐蚀，这给石油行业带来了巨大的职业和运营风险以及相应的经济损失。本研究旨在评估在碳钢试片上添加乙酸盐、丁酸盐、乳酸盐、丙酸盐和石油对生物膜结构的影响，以及对石油生产水中浮游和固着群落中总细菌和 SRB 多样性的影响。分析了生物膜形态、化学成分、平均粗糙度和微生物多样性。在所有碳源中，均检测到致密生物膜的形成，没有形态和/或微生物密度差异，大多数细胞以单个棒状形式存在。浮游群落中细菌物种的多样性和丰富度指数大于生物膜。地杆菌属是最丰富的属，超过 85%的 SRB 物种存在于所有处理中。功能预测图谱表明，浮游群落中观察到的属与硫酸盐、亚硫酸盐、单质硫和其他硫化合物的还原有关，但丰度在不同处理之间存在差异。对于生物膜，与对照相比，油处理的预测功能图谱变化最大，而对于浮游群落，所有碳源的添加都干扰了预测功能图谱。因此，尽管不同碳源对生产水的补充不会改变生物膜的结构和形态，但会改变 SRB 的分类组成和生物膜及浮游细菌群落的功能图谱。
