一株不动杆菌（Acinetobacter sp. SM04）合成的新型酶负责降解玉米赤霉烯酮。
A novel enzyme synthesized by Acinetobacter sp. SM04 is responsible for zearalenone biodegradation.
机构信息
School of Food Science and Engineering, South China University of Technology, Wu Shan, Guangzhou, P. R. China.
South China Institute of Collaborative Innovation, Dongguan, Guangdong, P. R. China.
出版信息
Biosci Biotechnol Biochem. 2022 Jan 24;86(2):209-216. doi: 10.1093/bbb/zbab204.
Zearalenone (ZEA), a nonsteroidal estrogenic mycotoxin produced by multiple Fusarium species, contaminates cereals and threatens the health of both humans and animals by inducing hepatotoxicity, immunotoxicity, and genotoxicity. A new alkali tolerant enzyme named Ase, capable of degrading ZEA without H2O2, was derived from Acinetobacter sp. SM04 in this study. The Ase gene shares 97% sequence identity with hypothetical proteins from Acinetobacter pittii strain WCHAP 100004 and YMC 2010/8/T346 and Acinetobacter calcoaceticus PHEA-2, respectively. Based on the Acinetobacter genus database, the gene encoding Ase was cloned and extracellularly expressed in Escherichia coli BL21. After degrading 88.4% of ZEA (20 µg/mL), it was confirmed through MCF-7 cell proliferation assays that Ase can transform ZEA into a nonestrogenic toxic metabolite. Recombinant Ase (molecular weight: 28 kDa), produced by E. coli BL21/pET32a(+)-His-Ase, was identified as an oxygen-utilizing and cytochrome-related enzyme with optimal activity at 60 °C and pH 9.0.
玉米赤霉烯酮（ZEA）是一种由多种镰刀菌产生的非甾体类雌激素真菌毒素，它污染了谷物，通过诱导肝毒性、免疫毒性和遗传毒性，威胁着人类和动物的健康。本研究从不动杆菌属 SM04 中分离得到一种新的耐碱酶 Ase，它能够在不使用 H2O2 的情况下降解 ZEA。Ase 基因与来自鲍氏不动杆菌 WCHAP 100004 和 YMC 2010/8/T346 以及醋酸钙不动杆菌 PHEA-2 的假定蛋白的序列具有 97%的同源性。根据不动杆菌属数据库，克隆并在大肠杆菌 BL21 中外源表达了编码 Ase 的基因。在降解 88.4%的 ZEA（20μg/mL）后，通过 MCF-7 细胞增殖试验证实 Ase 可以将 ZEA 转化为非雌激素毒性代谢物。由大肠杆菌 BL21/pET32a(+)-His-Ase 产生的重组 Ase（分子量：28kDa）被鉴定为一种需氧和细胞色素相关的酶，其最适活性在 60°C 和 pH9.0。
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