鉴定转座子插入突变，包括 rnpB::Tn，该突变消除了枯草芽孢杆菌中葡萄糖诱导 sigX 编码细胞外功能σ因子的作用。

Identification of transposon-inserted mutations including rnpB::Tn that abolished glucose induction of sigX encoding extracytoplasmic function-sigma factor in Bacillus subtilis.

机构信息

Institute of Oceanic Research and Development, Tokai University, Orido, Shizuoka, Japan.

出版信息

Biosci Biotechnol Biochem. 2022 Jan 24;86(2):282-285. doi: 10.1093/bbb/zbab211.

Abstract

We investigated the regulators of the glucose induction (GI) of the ECF-sigma genes sigX/M. During further screening of transposon-inserted mutants, we identified several regulators including an RNA component of RNase P (rnpB), which is required for tRNA maturation. A depletion of rnpB is known to trigger the stringent response. We showed evidence that the stringent response inhibited GI of sigX/M.

摘要

我们研究了调节外切体σ基因 sigX/M 的葡萄糖诱导（GI）的因素。在进一步筛选转座子插入突变体时，我们鉴定了几个调节因子，包括核糖核酸酶 P 的 RNA 成分（rnpB），其对于 tRNA 的成熟是必需的。rnpB 的耗竭已知会引发严谨反应。我们的研究结果表明，严谨反应抑制了 sigX/M 的 GI。