Laboratory of Molecular and Chemical Cell Biology, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan.
Hiroshima Research Center for Healthy Aging (HiHA), Hiroshima University, Higashi-Hiroshima, Japan.
Biosci Biotechnol Biochem. 2022 Jan 24;86(2):254-259. doi: 10.1093/bbb/zbab212.
Kinesin-5 family proteins are essential for bipolar spindle assembly to ensure mitotic fidelity. Here, we demonstrate evolutionary functional conservation of kinesin-5 between human and fission yeast. Human Eg5 expressed in the nucleus replaces fission yeast counterpart Cut7. Intriguingly, Eg5 overproduction results in cytotoxicity. This phenotype provides a useful platform for the development of novel kinesin-5 inhibitors as anticancer drugs.
驱动蛋白-5 家族蛋白对于双极纺锤体的组装至关重要，以确保有丝分裂的保真度。在这里，我们证明了人类和裂殖酵母之间驱动蛋白-5 的进化功能保守性。在核内表达的人源 Eg5 可替代裂殖酵母的 Cut7。有趣的是，Eg5 的过表达会导致细胞毒性。这种表型为开发新型驱动蛋白-5 抑制剂作为抗癌药物提供了一个有用的平台。
