Temperate Tree Fruit and Vegetable Research Unit, USDA-ARS, 5230 Konnowac Pass Road, Wapato, WA 98951, USA.
Temperate Tree Fruit and Vegetable Research Unit, USDA-ARS, Prosser, WA 99350, USA.
Environ Entomol. 2022 Feb 16;51(1):94-107. doi: 10.1093/ee/nvab133.
Insects harbor bacterial endosymbionts that provide their hosts with nutritional benefit or with protection against natural enemies, plant defenses, insecticides, or abiotic stresses. We used directed sequencing of 16S rDNA to identify and compare endosymbionts of Bactericera maculipennis (Crawford) and the western, central, and northwestern haplotypes of B. cockerelli (Šulc) (Hemiptera: Psylloidea: Triozidae). Both species are native to North America, are known to harbor the plant pathogen 'Candidatus Liberibacter solanacearum' and develop on shared host plants within the Convolvulaceae. The Old-World species Heterotrioza chenopodii (Reuter) (Psylloidea: Triozidae), now found in North America, was included as an outgroup. 16S sequencing confirmed that both Bactericera species harbor 'Candidatus Liberibacter solanacearum' and revealed that both species harbor unique strains of Wolbachia and Sodalis. However, the presence of Wolbachia and Sodalis varied among haplotypes of B. cockerelli. The central and western haplotypes harbored the same strains of Wolbachia, which was confirmed by Sanger sequencing of the wsp and ftsZ genes. Wolbachia was also detected in very low abundance from the northwestern haplotype by high-throughput sequencing of 16S but was not detected from this haplotype by PCR screening. The northwestern and central haplotypes also harbored Sodalis, which was not detected in the western haplotype. Heterotrioza chenopodii harbored an entirely different community of potential endosymbionts compared with the Bactericera spp. that included Rickettsia and an unidentified bacterium in the Enterobacteriaceae. Results of this study provide a foundation for further research on the interactions between psyllids and their bacterial endosymbionts.
昆虫体内携带有细菌内共生体，这些共生体为宿主提供营养益处或抵御天敌、植物防御、杀虫剂或非生物胁迫。我们使用 16S rDNA 定向测序来鉴定和比较 Bactericera maculipennis (Crawford) 和 B. cockerelli (Šulc)（半翅目：蚜科：瘿蚊科）的西部、中部和西北部单倍型的内共生体。这两个物种都原产于北美洲，已知携带植物病原体“Candidatus Liberibacter solanacearum”，并在旋花科的共享宿主植物上发育。旧世界物种 Heterotrioza chenopodii (Reuter)（半翅目：蚜科：瘿蚊科），现在在北美洲发现，被作为外群包含在内。16S 测序证实，这两个 Bactericera 物种都携带“Candidatus Liberibacter solanacearum”，并揭示这两个物种都携带有独特的沃尔巴克氏体和索达利氏菌菌株。然而，沃尔巴克氏体和索达利氏菌的存在在 B. cockerelli 的不同单倍型中有所不同。中部和西部单倍型携带相同的沃尔巴克氏体菌株，这通过 wsp 和 ftsZ 基因的 Sanger 测序得到了证实。通过 16S 的高通量测序，在西北单倍型中也检测到了极低丰度的沃尔巴克氏体，但通过 PCR 筛选并未从该单倍型中检测到。西北和中部单倍型也携带有索达利氏菌，而西部单倍型中则没有检测到。与 Bactericera spp. 相比，Heterotrioza chenopodii 体内携带的潜在共生体群落完全不同，其中包括 Rickettsia 和肠杆菌科中的一种未鉴定细菌。本研究的结果为进一步研究叶蝉及其细菌内共生体之间的相互作用提供了基础。
