Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID 83844, U.S.A.
United States Department of Agriculture-Agricultural Research Service, Temperate Tree Fruit and Vegetable Research Unit, Wapato, WA 98951, U.S.A.
Plant Dis. 2021 Sep;105(9):2560-2566. doi: 10.1094/PDIS-10-20-2240-RE. Epub 2021 Oct 14.
The potato psyllid (Šulc) (Hemiptera: Triozidae) is a pest of solanaceous crops (order Solanales), including potato ( L.) and tomato ( L.). Feeding by high populations of nymphs causes psyllid yellows while adults and nymphs are vectors of the plant pathogen ' Liberibacter solanacearum'. Foliar symptoms that were consistent with either ' L. solanacearum' infection or psyllid yellows were observed in 2019 on tomatillo ( Brot.; family Solanaceae) grown within an experimental plot located near Saltillo, Mexico. This study had three primary objectives: 9i) determine whether the foliar symptoms observed on tomatillo were associated with ' L. solanacearum' infection, (ii) identify the haplotypes of ' L. solanacearum' and potato psyllids present in the symptomatic plot, and (iii) use gut content analysis to infer the plant sources of ' L. solanacearum'-infected psyllids. Results confirmed that 71% of symptomatic plants and 71% of psyllids collected from the plants were infected with ' L. solanacearum'. The detection of ' L. solanacearum' in plants and psyllids and the lack of nymphal populations associated with psyllid yellows strongly suggests that the observed foliar symptoms were caused by ' L. solanacearum' infection. All infected plants and insects harbored the more virulent ' L. solanacearum' haplotype B but one psyllid was also coinfected with haplotype A. The potato psyllids were predominantly of the central haplotype but one psyllid was identified as the western haplotype. Molecular gut content analysis of psyllids confirmed the movement of psyllids between noncrop habitats and tomatillo and indicated that ' L. solanacearum' infection of psyllids was associated with increased plant diversity in their diet.
马铃薯木虱 (Šulc)（半翅目：粉虱科）是茄科作物（茄目）的害虫，包括马铃薯 (L.) 和番茄 (L.)。若若虫数量较高，会引起木虱黄化，而成虫和若虫则是植物病原菌 'Liberibacter solanacearum' 的传播媒介。2019 年，在墨西哥萨利托附近的一个实验田中种植的树番茄（Brot.；茄科）叶片出现了与 'L. solanacearum' 感染或木虱黄化一致的症状。本研究有三个主要目标：i）确定在树番茄上观察到的叶片症状是否与 'L. solanacearum' 感染有关，ii）鉴定该症状样本地带的 'L. solanacearum' 和马铃薯木虱的单倍型，以及 iii）使用肠道内容物分析推断感染 'L. solanacearum' 的木虱的植物来源。结果证实，71%的病株和从病株上采集的 71%的木虱感染了 'L. solanacearum'。在植物和木虱中检测到 'L. solanacearum'，且没有与木虱黄化相关的若虫种群，这强烈表明观察到的叶片症状是由 'L. solanacearum' 感染引起的。所有感染的植物和昆虫都携带了更具毒性的 'L. solanacearum' 单倍型 B，但有一只木虱也同时感染了单倍型 A。马铃薯木虱主要是中源单倍型，但有一只木虱被鉴定为西方单倍型。木虱肠道内容物的分子分析证实了木虱在非作物生境和树番茄之间的移动，并表明木虱感染 'L. solanacearum' 与其饮食中植物多样性的增加有关。
