College of Chemistry, Chemical Engineering and Materials Science - Soochow University, China.
Xbiome Co. Ltd. - Shenzhen, China.
Food Res Int. 2021 Dec;150(Pt A):110743. doi: 10.1016/j.foodres.2021.110743. Epub 2021 Oct 12.
The search for life/health quality has driven the search for a better understanding of food components on the overall individual health, which turns to be intrinsically related to the digestive system. In vitro digestion models are considered an alternative for the in vivo studies for a variety of practical reasons, but further research is still needed concerning the colon model establishment. An effective in vitro colon model should consider all unit operations and transport phenomena, together with chemical and biochemical reactions, material handling and reactor design. Due to the different techniques and dependence on the donor microbiota, it is difficult to obtain a standard protocol with results reproductible in time and space. Furthermore, the colon model should be fed with a representative substrate, thus what happens in upper digestion tract and absorption prior to colon is also of crucial importance. Essentially, there are two ways to think about how to achieve a good and useful in vitro colon model: a complex biomimetic system that provides results comparable with the in vivo studies or a simple system, that despite the fact it could not give physiologically relevant data, it is sufficient to understand the fate of some specific components.
对生命/健康质量的追求促使人们更好地了解食物成分对整体个体健康的影响，而这又与消化系统密切相关。由于各种实际原因，体外消化模型被认为是体内研究的一种替代方法，但关于结肠模型的建立仍需要进一步研究。一个有效的体外结肠模型应考虑所有单元操作和传质现象，以及化学反应、物料处理和反应器设计。由于不同的技术和对供体微生物区系的依赖，很难获得具有可重复性的标准方案。此外，结肠模型应该用代表性的底物进行喂养，因此在上消化道发生的情况以及在进入结肠之前的吸收过程也至关重要。从本质上讲，有两种方法可以考虑如何构建一个良好且有用的体外结肠模型：一种是提供与体内研究结果可比的复杂仿生系统，另一种是提供尽管不能提供生理相关数据但足以理解某些特定成分命运的简单系统。
