白藜芦醇对小鼠、人毛囊及毛乳头细胞的促毛发生长作用
Hair Growth-Promoting Effect of Resveratrol in Mice, Human Hair Follicles and Dermal Papilla Cells.
作者信息
Zhang Yuting, Ni Chunya, Huang Yan, Tang Yulong, Yang Kai, Shi Xiangguang, Zhang Yue, Li Zheng, Wang Ji'an, Zhu Yifei, Li Haiyang, Ma Yanyun, Lin Jinran, Wang Jiucun, Liu Qingmei, Wu Wenyu
机构信息
State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.
Department of Dermatology, Jing'an District Central Hospital, Shanghai, People's Republic of China.
出版信息
Clin Cosmet Investig Dermatol. 2021 Nov 27;14:1805-1814. doi: 10.2147/CCID.S335963. eCollection 2021.
BACKGROUND
Oxidative damage has been found in various types of hair loss. As a polyphenolic phytoalexin, resveratrol (RSV) is known as an antioxidant, anti-inflammatory and anti-apoptotic agent.
OBJECTIVE
Thus, we aim to examine the effects of RSV on hair growth.
METHODS
In vivo C57BL/6 mice were used to evaluate the effects of RSV on hair cycle, hair length, skin thickness, hair follicle diameter, hair cycle score and the percentage of hair cycle stage. Then hair shaft length and hair cycle were evaluated by human hair follicles (HFs) ex vivo. The proliferative activities of human dermal papilla cells (hDPCs) cultured in vitro with RSV were assessed using RTCA. The ability of RSV to protect hDPCs against HO-induced oxidative damage is examined by a ROS assay kit.
RESULTS
Topical application of RSV significantly promoted hair growth and stimulated the transition of hair cycle from telogen into the anagen phase on shaved C57BL/6 mice. Ex vivo experiments showed that RSV increased the hair shaft length of HFs and delayed the entry into catagen. In vitro experiments indicated that RSV proliferated hDPCs and prevented hDPCs from oxidative damage caused by HO.
CONCLUSION
RSV can promote hair growth and may be a potential candidate for the treatment of hair loss.
背景
在各种类型的脱发中均发现了氧化损伤。白藜芦醇（RSV）作为一种多酚类植物抗毒素，是一种抗氧化、抗炎和抗凋亡剂。
目的
因此，我们旨在研究RSV对毛发生长的影响。
方法
在体内实验中，使用C57BL/6小鼠评估RSV对毛发周期、毛发长度、皮肤厚度、毛囊直径、毛发周期评分及毛发周期阶段百分比的影响。然后在体外用人毛囊评估毛干长度和毛发周期。使用实时无标记细胞分析技术评估体外培养的人真皮乳头细胞（hDPCs）与RSV共同培养时的增殖活性。通过活性氧检测试剂盒检测RSV保护hDPCs免受过氧化氢（HO）诱导的氧化损伤的能力。
结果
在剃毛的C57BL/6小鼠上局部应用RSV可显著促进毛发生长，并刺激毛发周期从休止期转变为生长期。体外实验表明，RSV增加了毛囊的毛干长度并延迟了进入退行期。体外实验表明，RSV使hDPCs增殖，并防止hDPCs受到HO引起的氧化损伤。
结论
RSV可促进毛发生长，可能是治疗脱发的潜在候选药物。
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