Boisvert William A, Yu Miri, Choi Youngbin, Jeong Gi Hee, Zhang Yi-Lin, Cho Sunghun, Choi Changsun, Lee Sanghyun, Lee Bog-Hieu
John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, 96813, USA.
Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Korea.
BMC Complement Altern Med. 2017 Feb 13;17(1):109. doi: 10.1186/s12906-017-1624-4.
Geranium sibiricum L. has been used as a medicinal plant to treat diarrhea, bacterial infection, and cancer in Bulgaria, Peru, and Korea. However, its hair growth-promoting effect was not investigated so far. This study examined the effects of Geranium sibiricum L. extract (GSE) on hair growth, using in vitro and in vivo models.
Antioxidant, proliferation and migration assay of GSE was performed with human dermal papilla cells (hDPCs). Hair-growth promoting effect was measured in animal model. Relative expression of interleukin-1, vascular endothelial growth factor, hepatocyte growth factor, and transforming growth factor beta 1 was determined by real time RT-PCR. Expression of Ki-67 and stem cell factor were analyzed by immunohistochemistry.
GSE treatment proliferated and migrated human dermal papilla cells (hDPCs) more than treatment of 10 μM minoxidil. GSE significantly stimulated the expression of Ki-67 protein and the mRNA levels of hepatocyte growth factor and vascular endothelial growth factor in hDPCs. Topical application of 1,000 ppm GSE for 3 weeks promoted more significant hair growth on shaved C57BL/6 mice than did 5% minoxidil. The histological morphology of hair follicles demonstrated an active anagen phase with the induction of stem cell factor. GSE treatment significantly reduced the number of mast cells and the expression of transforming growth factor beta 1 in mouse skin tissues.
These results demonstrated that GSE promotes hair growth in vitro and in vivo by regulating growth factors and the cellular response.
在保加利亚、秘鲁和韩国，老鹳草已被用作药用植物来治疗腹泻、细菌感染和癌症。然而，其促进头发生长的作用迄今尚未得到研究。本研究使用体外和体内模型，检测了老鹳草提取物（GSE）对头发生长的影响。
对人毛乳头细胞（hDPCs）进行GSE的抗氧化、增殖和迁移测定。在动物模型中测量促进头发生长的作用。通过实时逆转录聚合酶链反应（RT-PCR）测定白细胞介素-1、血管内皮生长因子、肝细胞生长因子和转化生长因子β1的相对表达。通过免疫组织化学分析Ki-67和干细胞因子的表达。
与10μM米诺地尔处理相比，GSE处理使hDPCs增殖和迁移更多。GSE显著刺激hDPCs中Ki-67蛋白的表达以及肝细胞生长因子和血管内皮生长因子的mRNA水平。在剃毛的C57BL/6小鼠上局部应用1000ppm GSE 3周，比5%米诺地尔促进头发生长更显著。毛囊的组织形态学显示诱导干细胞因子后处于活跃的生长期。GSE处理显著减少了小鼠皮肤组织中肥大细胞的数量和转化生长因子β1的表达。
这些结果表明，GSE通过调节生长因子和细胞反应在体外和体内促进头发生长。
