Identification of Novel Type Three Secretion System (T3SS) Inhibitors by Computational Methods and Anti- Evaluations.

Three type III secretion system (T3SS) inhibitors (compounds 5, 19, and 32) were identified by virtual screening and biological evaluation. These three compounds were evaluated against a panel of species strains including , , , , and , and their minimum inhibitory concentrations ranged from 1 to 53 μg/ml. Especially, these compounds showed comparable activity as the of the positive control gatifloxacin towards . The present results suggest that these new T3SS inhibitors could be used as a potential lead molecule for drug development of anti-.