Abdelhafiz Yousri, Fernandes Jorge M O, Stefani Erika, Albanese Davide, Donati Claudio, Kiron Viswanath
Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.
Unit of Computational Biology, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.
Front Microbiol. 2021 Nov 16;12:773351. doi: 10.3389/fmicb.2021.773351. eCollection 2021.
Fish are widely exposed to higher microbial loads compared to land and air animals. It is known that the microbiome plays an essential role in the health and development of the host. The oral microbiome is vital in females of different organisms, including the maternal mouthbrooding species such as Nile tilapia (). The present study reports for the first time the microbial composition in the buccal cavity of female and male Nile tilapia reared in a recirculating aquaculture system. Mucus samples were collected from the buccal cavity of 58 adult fish (∼1 kg), and 16S rRNA gene amplicon sequencing was used to profile the microbial communities in females and males. The analysis revealed that opportunistic pathogens such as sp. were less abundant in the female buccal cavity. The power play of certain bacteria such as , Acidobacteria (GP4 and GP6), and Saccharibacteria that have known metabolic advantages was evident in females compared to males. Association networks inferred from relative abundances showed few microbe-microbe interactions of opportunistic pathogens in female fish. The findings of opportunistic bacteria and their interactions with other microbes will be valuable for improving Nile tilapia rearing practices. The presence of bacteria with specific functions in the buccal cavity of female fish points to their ability to create a protective microbial ecosystem for the offspring.
与陆地和空中动物相比，鱼类广泛暴露于更高的微生物负荷之下。众所周知，微生物群在宿主的健康和发育中起着至关重要的作用。口腔微生物群在不同生物的雌性个体中至关重要，包括尼罗罗非鱼等口育雌鱼物种。本研究首次报告了在循环水养殖系统中饲养的尼罗罗非鱼雌鱼和雄鱼口腔中的微生物组成。从58条成年鱼（约1千克）的口腔中采集黏液样本，并使用16S rRNA基因扩增子测序对雌鱼和雄鱼的微生物群落进行分析。分析表明，诸如 属等机会致病菌在雌鱼口腔中的丰度较低。与雄鱼相比，某些具有已知代谢优势的细菌，如 、酸杆菌（GP4和GP6）和糖菌在雌鱼中的作用更为明显。从相对丰度推断出的关联网络显示，雌鱼中机会致病菌的微生物-微生物相互作用较少。机会致病菌及其与其他微生物相互作用的研究结果对于改进尼罗罗非鱼的养殖实践具有重要价值。雌鱼口腔中具有特定功能的细菌的存在表明它们有能力为后代创造一个保护性的微生物生态系统。
