Rui Cun, Zhang Yuexin, Fan Yapeng, Han Mingge, Dai Maohua, Wang Qinqin, Chen Xiugui, Lu Xuke, Wang Delong, Wang Shuai, Gao Wenwei, Yu John Z, Ye Wuwei
State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, School of Agricultural Sciences, Zhengzhou University/Key Laboratory for Cotton Genetic Improvement, MOA, Anyang, China.
Engineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, Ürümqi, China.
Front Plant Sci. 2021 Nov 11;12:772123. doi: 10.3389/fpls.2021.772123. eCollection 2021.
is a cultivated cotton not only known for producing superior fiber but also for its salt and alkaline resistance. Here, we used Whole Genome Bisulfite Sequencing (WGBS) technology to map the cytosine methylation of the whole genome of the hypocotyl at single base resolution. The methylation sequencing results showed that the mapping rates of the three samples were 75.32, 77.54, and 77.94%, respectively. In addition, the Bisulfite Sequence (BS) conversion rate was 99.78%. Approximately 71.03, 53.87, and 6.26% of the cytosine were methylated at CG, CHG, and CHH sequence contexts, respectively. A comprehensive analysis of DNA methylation and transcriptome data showed that the methylation level of the promoter region was a positive correlation in the CHH context. Saline-alkaline stress was related to the methylation changes of many genes, transcription factors (TFs) and transposable elements (TEs), respectively. We explored the regulatory mechanism of DNA methylation in response to salt and alkaline stress during cotton hypocotyl elongation. Our data shed light into the relationship of methylation regulation at the germination stage of hypocotyl cell elongation and salt-alkali treatment. The results of this research help understand the early growth regulation mechanism of in response to abiotic stress.
是一种栽培棉花，不仅以生产优质纤维而闻名，还具有耐盐碱能力。在这里，我们使用全基因组亚硫酸氢盐测序（WGBS）技术，以单碱基分辨率绘制下胚轴全基因组的胞嘧啶甲基化图谱。甲基化测序结果表明，三个样本的映射率分别为75.32%、77.54%和77.94%。此外，亚硫酸氢盐序列（BS）转化率为99.78%。分别约有71.03%、53.87%和6.26%的胞嘧啶在CG、CHG和CHH序列背景下发生甲基化。对DNA甲基化和转录组数据的综合分析表明，启动子区域的甲基化水平在CHH背景下呈正相关。盐碱胁迫分别与许多基因、转录因子（TFs）和转座元件（TEs）的甲基化变化有关。我们探索了棉花下胚轴伸长过程中DNA甲基化响应盐碱胁迫的调控机制。我们的数据揭示了下胚轴细胞伸长萌发阶段甲基化调控与盐碱处理之间的关系。本研究结果有助于理解棉花响应非生物胁迫的早期生长调控机制。
