Gao Zhao-Wei, Liu Chong, Yang Lan, Chen Hao-Chuan, Yang Long-Fei, Zhang Hui-Zhong, Dong Ke
Department of Clinical Laboratory, The Second Affiliated Hospital, Air Force Medical University, Xi'an, China.
Front Genet. 2021 Nov 17;12:728200. doi: 10.3389/fgene.2021.728200. eCollection 2021.
To investigate the expression levels and prognostic value of CD73 in lung cancer. And moreover, to identify the effect and potential mechanism of CD73 on lung cancer cells proliferation and migration. CD73 expression levels in lung cancer were analyzed base on GEPIA2 and GEO database. GEPIA2 and Kaplan-Meier Plotter (KM Plotter) was used to analyzed the correlation between CD73 expression and prognosis. GEO dataset were analyzed via GEO2R. CD73 overexpression cell model was construction via recombinant lentivirus transfection into A549 and NCI-H520 cells. CCK8 assay were used to investigate cells proliferation. Migration and invasion ability were evaluated by scratch and transwell methods. Base on GEPIA2, GSE32683, GSE116959 and GSE37745 dataset, we found that CD73 expression were significant higher in tumor tissues of lung adenocarcinoma (LUAD) compared with that in non-tumor normal tissues and in lung squamous cell carcinoma (LUSC), while there were no significant difference of CD73 expression between LUSC and normal control tissues. Interestingly, a high CD73 level predict poor overall survival (OS) of LUSC. However, GEPIA2 and KM plotter showed the opposite conclusion of prognostic value of CD73 in LUAD. By using cell experiments, we found that CD73 overexpression promoted proliferation and migration of LUAD A549 cells. However, there was no significant effect of CD73 overexpression on LUSC NCI-H520 cells. Furthermore, CD73 overexpression facilitates epithelial to mesenchymal transition (EMT) progression of A549 cells. In conclusion, our results indicated that CD73 expression were increased in LUAD and might be an poor prognostic marker for LUSC patients. CD73 play an important role in LUAD cells proliferation and migration. These data allowed to support CD73 as a therapeutic target for LUAD.
研究CD73在肺癌中的表达水平及预后价值。此外，确定CD73对肺癌细胞增殖和迁移的影响及潜在机制。基于GEPIA2和GEO数据库分析肺癌中CD73的表达水平。使用GEPIA2和Kaplan-Meier Plotter（KM Plotter）分析CD73表达与预后之间的相关性。通过GEO2R分析GEO数据集。通过重组慢病毒转染A549和NCI-H520细胞构建CD73过表达细胞模型。使用CCK8法研究细胞增殖。通过划痕和Transwell法评估迁移和侵袭能力。基于GEPIA2、GSE32683、GSE116959和GSE37745数据集，我们发现肺腺癌（LUAD）肿瘤组织中CD73的表达明显高于非肿瘤正常组织和肺鳞状细胞癌（LUSC），而LUSC与正常对照组织之间CD73表达无显著差异。有趣的是，高CD73水平预示LUSC患者总生存期（OS）较差。然而，GEPIA2和KM Plotter显示CD73在LUAD中的预后价值结论相反。通过细胞实验，我们发现CD73过表达促进LUAD A549细胞的增殖和迁移。然而，CD73过表达对LUSC NCI-H520细胞无显著影响。此外，CD73过表达促进A549细胞的上皮-间质转化（EMT）进程。总之，我们的结果表明LUAD中CD73表达增加，可能是LUSC患者预后不良的标志物。CD73在LUAD细胞增殖和迁移中起重要作用。这些数据支持将CD73作为LUAD的治疗靶点。
