Gansbacher B, Levinson A I
Immunology. 1986 Jun;58(2):191-6.
Recent studies indicate that human T cells expressing the T4 determinant are comprised of functionally distinct subsets. We investigated if activation of OKT4+ cells with the mitogen PHA affected their ability to regulate the proliferation and polyclonal differentiation of autologous B cells. OKT4+ cells were preactivated with PHA and then cocultured with autologous B cells in the presence or absence of PWM. B-cell proliferation and differentiation to immunoglobulin-secreting cells (IgSC) were assessed by [3H]thymidine incorporation and reverse haemolytic plaque assay, respectively. In the absence of PWM, the PHA-activated OKT4+ cells demonstrated radioresistant help and radiosensitive suppression of IgSC responses. Addition of PWM to cocultures of irradiated PHA preactivated OKT4 cells and autologous B cells resulted in further suppression of IgSC responses, suggesting that PWM activated yet another suppression mechanism. Addition of PWM caused diminished B-cell proliferation as well. These data demonstrate functional heterogeneity within the OKT4 subset, and suggest that the particular immunoregulatory activity displayed is influenced by the state and mode of activation of these cells.
近期研究表明，表达T4决定簇的人类T细胞由功能不同的亚群组成。我们研究了用丝裂原PHA激活OKT4 +细胞是否会影响其调节自体B细胞增殖和多克隆分化的能力。用PHA预激活OKT4 +细胞，然后在有或无PWM的情况下与自体B细胞共培养。分别通过[3H]胸苷掺入和反向溶血空斑试验评估B细胞增殖和向免疫球蛋白分泌细胞（IgSC）的分化。在没有PWM的情况下，PHA激活的OKT4 +细胞表现出对IgSC反应的抗辐射辅助作用和辐射敏感抑制作用。将PWM添加到经照射的PHA预激活的OKT4细胞与自体B细胞的共培养物中导致对IgSC反应的进一步抑制，这表明PWM激活了另一种抑制机制。添加PWM也导致B细胞增殖减少。这些数据证明了OKT4亚群内的功能异质性，并表明所显示的特定免疫调节活性受这些细胞的激活状态和模式影响。
