Luo Wenjie, Wang Jin, Dai Xiaoyan, Zhang Hailiang, Qu Yuanyuan, Xiao Wenjun, Ye Dingwei, Zhu Yiping
Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China.
Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
Front Oncol. 2021 Nov 19;11:754845. doi: 10.3389/fonc.2021.754845. eCollection 2021.
This study aimed to explore the role of in CD8+ T cell tumor infiltration and outcomes of bladder cancer (BLCA) patients after immunotherapy.
The correlation between expression and tumor infiltration of immune cells was analyzed using the Tumor Immune Estimation Resource database. The prognostic significance of in BLCA was analyzed using Kaplan-Meier curves. Immunohistochemistry was used to detect CD8+ T cell infiltration in tumors with high and low expression obtained from patients at the Fudan University Shanghai Cancer Center. The relationships between immune checkpoint genes and immune response were analyzed using The Cancer Genome Atlas and IMvigor 210 cohorts. The molecular functions, cellular components, and biological processes involving were explored using Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment pathway analyses.
The expression level of was significantly correlated with the infiltration of CD8+ T cells in BLCA tumors (r = 0.192, P = 2.22e-04). Elevated was associated with suppressed tumor progression and better outcomes for BLCA patients. The higher expression level of predicted better immunotherapeutic responses and was associated with higher expression levels of core immune checkpoint genes, including , , , and , compared with the low expression group.
This study demonstrated for the first time that elevated correlated significantly with CD8+ T cell infiltration and contributed to better immunotherapeutic responses in BLCA patients. Furthermore, serves as a novel biomarker for predicting patient outcomes after immunotherapeutic treatments, which may improve the development of individualized immunotherapy for BLCA.
本研究旨在探讨[具体内容缺失]在CD8 + T细胞肿瘤浸润及膀胱癌（BLCA）患者免疫治疗后预后中的作用。
使用肿瘤免疫评估资源数据库分析[具体内容缺失]表达与免疫细胞肿瘤浸润之间的相关性。使用Kaplan - Meier曲线分析[具体内容缺失]在BLCA中的预后意义。采用免疫组织化学方法检测复旦大学附属肿瘤医院患者中[具体内容缺失]高表达和低表达肿瘤中的CD8 + T细胞浸润情况。使用癌症基因组图谱和IMvigor 210队列分析免疫检查点基因与免疫反应之间的关系。通过京都基因与基因组百科全书和基因本体富集通路分析探索涉及[具体内容缺失]的分子功能、细胞成分和生物学过程。
[具体内容缺失]的表达水平与BLCA肿瘤中CD8 + T细胞的浸润显著相关（r = 0.192，P = 2.22e - 04）。[具体内容缺失]升高与BLCA患者肿瘤进展受抑制及更好的预后相关。与[具体内容缺失]低表达组相比，[具体内容缺失]较高的表达水平预示着更好的免疫治疗反应，且与包括[具体基因缺失]、[具体基因缺失]、[具体基因缺失]和[具体基因缺失]在内的核心免疫检查点基因的较高表达水平相关。
本研究首次证明[具体内容缺失]升高与CD8 + T细胞浸润显著相关，并有助于BLCA患者获得更好的免疫治疗反应。此外，[具体内容缺失]可作为预测免疫治疗后患者预后的新型生物标志物，这可能会改善BLCA个体化免疫治疗的发展。
