Li Suyi, Jiang Cong, Chen Hua, Zhang Lijuan, Ke Ling, Chen Xu, Lin Chentao
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, China.
Fujian Key Laboratory of Marine Enzyme Engineering, College of Biological Sciences and Engineering, Fuzhou University, Fuzhou, China.
Front Vet Sci. 2021 Nov 18;8:741242. doi: 10.3389/fvets.2021.741242. eCollection 2021.
Tumor necrosis factor (TNF) plays an important role in an inflammatory cytokine storm. Over-secretion of TNF by the host in response to infection aggravates the disease. TNF expression level is positively correlated with the mortality caused by some bacterial infections. Therefore, using TNF antibody may alleviate the inflammation to resist bacterial infections. The function of fish TNF-b antibody in bacterial infection is still unclear. In this study, infection models of FJ03-X2 strain with high pathogenicity and strong virulence were established in zebrafish () fibroblast cell line (ZF4 cells) and zebrafish. Zebrafish () gene was cloned and expressed by BL21 (DE3), and Zetnf-b polyclonal antibody was prepared. Pre-injection of Zetnf-b polyclonal antibody and AG-126 before infecting with could increase the survival rate of zebrafish by 36.6 and 46.7%, respectively. Pre-injection of Zetnf-b polyclonal antibody could effectively decrease the mortality of zebrafish infected by . Thus, TNF polyclonal antibody therapy could be considered as an effective strategy to control in fish.
肿瘤坏死因子（TNF）在炎症细胞因子风暴中起重要作用。宿主对感染的反应中TNF过度分泌会加重疾病。TNF表达水平与某些细菌感染导致的死亡率呈正相关。因此，使用TNF抗体可能减轻炎症以抵抗细菌感染。鱼类TNF-b抗体在细菌感染中的功能仍不清楚。在本研究中，在斑马鱼（）成纤维细胞系（ZF4细胞）和斑马鱼中建立了具有高致病性和强毒力的FJ03-X2菌株感染模型。斑马鱼（）基因通过BL21（DE3）克隆并表达，制备了Zetnf-b多克隆抗体。在感染前预先注射Zetnf-b多克隆抗体和AG-126可使斑马鱼的存活率分别提高36.6%和46.7%。预先注射Zetnf-b多克隆抗体可有效降低感染的斑马鱼的死亡率。因此，TNF多克隆抗体疗法可被视为控制鱼类中感染的有效策略。
