Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, Brazil.
Paulo Picanço School of Dentistry, Fortaleza, Brazil.
Biomed Res Int. 2021 Nov 25;2021:8414577. doi: 10.1155/2021/8414577. eCollection 2021.
To investigate physicochemical properties, dentin bonding, cytotoxicity, and in vivo pulp response of experimental self-adhesive composites tailored to direct pulp capping.
Experimental composites were prepared with beta-tricalcium phosphate and hydroxyapatite nanoparticles adsorbed with simvastatin and glutathione added at 0% (control resin), 1 wt% (Res 1%), and 10 wt% (Res 10%). A commercial light-curable calcium hydroxide (Ca(OH)) (Ultra-Blend Plus) was used as control material. The physicochemical properties investigated were flexural strength and modulus, calcium release, and degree of conversion. Dentin bonding was assessed by the push-out test. Proliferation and cell counting assays were performed to evaluate in vitro cytotoxicity using fluorescence microscopy. In vivo pulp capping was performed on molars of Wistar rats, which were euthanized after 14 days and evaluated by histological analysis.
No statistical difference was observed in flexural strength and cell viability ( > 0.05). Res 10% presented higher modulus than control resin and Ca(OH). Also, Res 10% attained statistically higher degree of conversion when compared to other experimental composites. Ca(OH) showed higher calcium release after 28 and 45 days of storage, with no statistical difference at 45 days to Res 10%. All experimental composites achieved significantly higher bond strength when compared to Ca(OH). While no significant difference was observed in the cell proliferation rates, resins at lower concentrations showed higher cell viability. In vivo evaluation of pulp response demonstrated no pulp damage with experimental composites.
The experimental composite investigated in this study achieved adequate physicochemical properties with minor in vivo pulpal inflammation and proved to be a valuable alternative for direct pulp capping.
研究经改良以用于直接盖髓的实验性自粘接复合材料的理化性能、牙本质粘接性能、细胞毒性和体内牙髓反应。
实验性复合材料中添加了吸附有辛伐他汀和谷胱甘肽的β-磷酸三钙和羟基磷灰石纳米颗粒，浓度分别为 0%（对照树脂）、1wt%（Res 1%）和 10wt%（Res 10%）。商用光固化氢氧化钙（Ultra-Blend Plus）作为对照材料。检测的理化性能包括弯曲强度和模量、钙释放和转化率。通过推出试验评估牙本质粘接性能。采用荧光显微镜进行细胞增殖和细胞计数检测，以评估体外细胞毒性。在 Wistar 大鼠磨牙上进行体内盖髓，14 天后处死大鼠并进行组织学分析。
弯曲强度和细胞活力（>0.05）无统计学差异。Res 10%的模量高于对照树脂和 Ca(OH)。与其他实验性复合材料相比，Res 10%的转化率也更高。Ca(OH)在储存 28 和 45 天后的钙释放量更高，但与 Res 10%相比，45 天后无统计学差异。与 Ca(OH)相比，所有实验性复合材料的粘结强度均显著提高。虽然细胞增殖率无显著差异，但低浓度的树脂具有更高的细胞活力。体内牙髓反应评估表明，实验性复合材料无牙髓损伤。
本研究中所研究的实验性复合材料具有足够的理化性能，对牙髓仅有轻微的炎症反应，是直接盖髓的一种有价值的替代方法。
