Gandolfi M G, Spagnuolo G, Siboni F, Procino A, Rivieccio V, Pelliccioni G A, Prati C, Rengo S
Dental School (Laboratory of Biomaterials and Oral Pathology), Department of Biomedical and NeuroMotor Sciences, University of Bologna, Bologna, Italy.
Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples "Federico II", Napoli, Italy.
Clin Oral Investig. 2015 Nov;19(8):2075-89. doi: 10.1007/s00784-015-1443-2. Epub 2015 Mar 31.
The aim was to test the properties of experimental calcium silicate/calcium phosphate biphasic cements with hydraulic properties designed for vital pulp therapy as direct pulp cap and pulpotomy.
CaSi-αTCP and CaSi-DCDP were tested for ion-releasing ability, solubility, water sorption, porosity, ability to nucleate calcium phosphates, and odontoblastic differentiation—alkaline phosphatase (ALP) and osteocalcin (OCN) upregulation—of primary human dental pulp cells (HDPCs).
The materials showed high Ca and OH release, high open pore volume and apparent porosity, and a pronounced ability to nucleate calcium phosphates on their surface. HDPCs treated with CaSi-αTCP showed a strong upregulation of ALP and OCN genes, namely a tenfold increase for OCN and a threefold increase for ALP compared to the control cells. Conversely, CaSi-DCDP induced a pronounced OCN gene upregulation but had no effect on ALP gene regulation.
Both cements showed high biointeractivity (release of Ca and OH ions) correlated with their marked ability to nucleate calcium phosphates. CaSi-αTCP cement proved to be a potent inducer of ALP and OCN genes as characteristic markers of mineralization processes normally poorly expressed by HDPCs.
Calcium silicate/calcium phosphate cements appear to be attractive new materials for vital pulp therapy as they may provide odontogenic/dentinogenic chemical signals for pulp regeneration and healing, and dentin formation in regenerative endodontics.
旨在测试具有用于活髓治疗的水硬性的实验性硅酸钙/磷酸钙双相水泥作为直接盖髓剂和牙髓切断术材料的性能。
对CaSi-αTCP和CaSi-DCDP进行离子释放能力、溶解度、吸水率、孔隙率、磷酸钙成核能力以及原代人牙髓细胞（HDPCs）的成牙本质细胞分化——碱性磷酸酶（ALP）和骨钙素（OCN）上调——的测试。
这些材料显示出高钙和氢氧根释放、高开放孔隙体积和表观孔隙率，以及在其表面显著的磷酸钙成核能力。用CaSi-αTCP处理的HDPCs显示出ALP和OCN基因的强烈上调，即与对照细胞相比，OCN增加了10倍，ALP增加了3倍。相反，CaSi-DCDP诱导了显著的OCN基因上调，但对ALP基因调控没有影响。
两种水泥都显示出高生物活性（钙和氢氧根离子释放），与其显著的磷酸钙成核能力相关。CaSi-αTCP水泥被证明是ALP和OCN基因的有效诱导剂，这是HDPCs通常表达较差的矿化过程的特征性标志物。
硅酸钙/磷酸钙水泥似乎是活髓治疗有吸引力的新材料，因为它们可能为牙髓再生和愈合以及再生牙髓病学中的牙本质形成提供牙源性/牙本质生成化学信号。
