College of Pharmacy, Linyi University, Linyi, Shandong, China.
Linyi Tumor Hospital, Linyi, Shandong, China.
J Diabetes Res. 2021 Nov 24;2021:7618166. doi: 10.1155/2021/7618166. eCollection 2021.
In this study, a diabetic kidney disease model was established by placing the test rats on a high-sugar/high-fat diet combined with streptozotocin induction. Histopathological examination (H&E, Masson, and PASM stain) showed pathological changes in the diabetic rat kidneys, in addition to fibrotic symptoms and collagen deposition. Immunohistochemistry and western blot analyses indicated that the diabetic condition significantly increased the expressions of fibrotic markers including collagen, -SMA, and fibronectin. The levels of cholesterol, triglyceride, and low-density lipoprotein were also increased in diabetic kidney disease (DKD) rat blood, while the level of high-density lipoprotein was decreased. The results of Oil red O staining experiments indicated that the kidneys of diabetic rats exhibited appreciable fat deposition, with high contents of triglyceride and cholesterol. To inhibit fibrosis and reduce fat deposition, low molecular weight fucoidan (LMWF) may be used. Based on PCR and western blot analyses, LMWF can regulate the expression levels of important lipid metabolism regulators, thereby impeding the development of kidney fibrosis. Through the vitro model, it also be indicated that LMWF could inhibit fibrosis process through regulating lipid metabolism which induced by palmitic acid.
在这项研究中，通过给予实验大鼠高糖/高脂肪饮食联合链脲佐菌素诱导建立了糖尿病肾病模型。组织病理学检查（H&E、Masson 和 PASM 染色）显示，糖尿病大鼠肾脏出现了病理性变化，此外还有纤维化症状和胶原沉积。免疫组织化学和 Western blot 分析表明，糖尿病状态显著增加了纤维化标志物的表达，包括胶原、-SMA 和纤维连接蛋白。糖尿病肾病（DKD）大鼠血液中的胆固醇、甘油三酯和低密度脂蛋白水平也升高，而高密度脂蛋白水平降低。油红 O 染色实验的结果表明，糖尿病大鼠的肾脏表现出明显的脂肪沉积，甘油三酯和胆固醇含量高。为了抑制纤维化和减少脂肪沉积，可以使用低分子量褐藻糖胶（LMWF）。基于 PCR 和 Western blot 分析，LMWF 可以调节重要脂质代谢调节剂的表达水平，从而阻碍肾脏纤维化的发展。通过体外模型，还表明 LMWF 可以通过调节棕榈酸诱导的脂质代谢来抑制纤维化过程。
