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本文引用的文献

1
Transcriptome-wide study in the green microalga Messastrum gracile SE-MC4 identifies prominent roles of photosynthetic integral membrane protein genes during exponential growth stage.在绿色微藻中进行转录组研究表明,在指数生长阶段,光合作用的整合膜蛋白基因发挥了重要作用。
Phytochemistry. 2021 Dec;192:112936. doi: 10.1016/j.phytochem.2021.112936. Epub 2021 Sep 9.
2
Double-high in palmitic and oleic acids accumulation in a non-model green microalga, Messastrum gracile SE-MC4 under nitrate-repletion and -starvation cultivations.硝酸盐充足和饥饿培养条件下非模式绿藻小新月菱形藻中棕榈酸和油酸的双重高积累。
Sci Rep. 2021 Jan 11;11(1):381. doi: 10.1038/s41598-020-79711-2.
3
Influence of nitrogen availability on biomass, lipid production, fatty acid profile, and the expression of fatty acid desaturase genes in Messastrum gracile SE-MC4.氮供应对纤细海链藻 SE-MC4 生物量、油脂生成、脂肪酸组成和脂肪酸去饱和酶基因表达的影响。
World J Microbiol Biotechnol. 2020 Jan 7;36(1):17. doi: 10.1007/s11274-019-2790-y.
4
Effects of Excess and Limited Phosphate on Biomass, Lipid and Fatty Acid Contents and the Expression of Four Fatty Acid Desaturase Genes in the Tropical Selenastraceaen Messastrum gracile SE-MC4.过量和有限磷酸盐对热带海洋蓝藻中拟菱形藻 SE-MC4 生物量、脂质和脂肪酸含量及四个脂肪酸去饱和酶基因表达的影响。
Appl Biochem Biotechnol. 2020 Apr;190(4):1438-1456. doi: 10.1007/s12010-019-03182-z. Epub 2019 Nov 28.
5
whole genome sequencing data of two mangrove-isolated microalgae from Terengganu coastal waters.来自登嘉楼沿海水域的两种红树林分离微藻的全基因组测序数据。
Data Brief. 2019 Oct 18;27:104680. doi: 10.1016/j.dib.2019.104680. eCollection 2019 Dec.
6
Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing.BGISEQ-500与Illumina HiSeq2500测序平台在古基因组测序方面的比较性能
Gigascience. 2017 Aug 1;6(8):1-13. doi: 10.1093/gigascience/gix049.
7
Full-length transcriptome assembly from RNA-Seq data without a reference genome.无参考基因组的 RNA-Seq 数据的全长转录组组装。
Nat Biotechnol. 2011 May 15;29(7):644-52. doi: 10.1038/nbt.1883.

指数生长期和平稳生长期下SE-MC4的转录组测序数据。

Transcriptomics sequencing data of SE-MC4 under exponential and stationary growth stages.

作者信息

Afifudeen C L Wan, Loh Saw Hong, Wong Li Lian, Aziz Ahmad, Takahashi Kazutaka, Wahid Mohd Effendy Abd, Cha Thye San

机构信息

Satreps-Cosmos Laboratory, Central Laboratory Complex, University Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia.

Institute of Marine Biotechnology, University Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia.

出版信息

Data Brief. 2021 Nov 19;39:107607. doi: 10.1016/j.dib.2021.107607. eCollection 2021 Dec.

DOI:10.1016/j.dib.2021.107607
PMID:34869809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8626828/
Abstract

SE-MC4 is a non-model microalga exhibiting superior oil-accumulating abilities. However, biomass production in SE-MC4 is limited due to low cell proliferation especially after prolonged cultivation under oil-inducing culture conditions. Present data consist of next generation RNA sequencing data of SE-MC4 under exponential and stationary growth stages. RNA of six samples were extracted and sequenced with insert size of 100 bp paired-end strategy using BGISEQ-500 platform to produce a total of 59.64 Gb data with 314 million reads. Sequences were filtered and assembled to form 53,307 number of gene sequences. Sequencing data were deposited in National Center for Biotechnology Information (NCBI) and can be accessed via BioProject ID PRJNA552165. This information can be used to enhance biomass production in SE-MC4 and other microalgae aimed towards improving biodiesel development.

摘要

SE-MC4是一种具有卓越油脂积累能力的非模式微藻。然而,由于细胞增殖率低,特别是在油脂诱导培养条件下长时间培养后,SE-MC4的生物量产量受到限制。现有数据包括SE-MC4在指数生长期和稳定生长期的下一代RNA测序数据。提取了六个样本的RNA,并使用BGISEQ-500平台采用100 bp插入片段大小的双末端策略进行测序,共产生了5.964Gb数据,包含3.14亿条 reads。对序列进行过滤和组装,形成了53307个基因序列。测序数据已存入美国国立生物技术信息中心(NCBI),可通过生物项目编号PRJNA552165访问。这些信息可用于提高SE-MC4和其他微藻的生物量产量,以促进生物柴油的发展。