Satreps-Cosmos Laboratory, Central Laboratory Complex, Universiti Malaysia Terengganu, 21030, Terengganu, Malaysia.
Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Terengganu, Malaysia.
Sci Rep. 2021 Jan 11;11(1):381. doi: 10.1038/s41598-020-79711-2.
Bioprospecting for biodiesel potential in microalgae primarily involves a few model species of microalgae and rarely on non-model microalgae species. Therefore, the present study determined changes in physiology, oil accumulation, fatty acid composition and biodiesel properties of a non-model microalga Messastrum gracile SE-MC4 in response to 12 continuous days of nitrate-starve (NS) and nitrate-replete (NR) conditions respectively. Under NS, the highest oil content (57.9%) was achieved despite reductions in chlorophyll content, biomass productivity and lipid productivity. However, under both NS and NR, palmitic acid and oleic acid remained as dominant fatty acids thus suggesting high potential of M. gracile for biodiesel feedstock consideration. Biodiesel properties analysis returned high values of cetane number (CN 61.9-64.4) and degree of unsaturation (DU 45.3-57.4) in both treatments. The current findings show the possibility of a non-model microalga to inherit superior ability over model species in oil accumulation for biodiesel development.
生物勘探主要针对少数几种模式微藻的生物柴油潜力，而很少涉及非模式微藻物种。因此，本研究分别确定了非模式微藻 Messastrum gracile SE-MC4 在硝酸盐饥饿（NS）和硝酸盐充足（NR）条件下连续 12 天的生理变化、油脂积累、脂肪酸组成和生物柴油特性。在 NS 条件下，尽管叶绿素含量、生物量生产力和脂质生产力降低，但仍达到了最高的油脂含量（57.9%）。然而，在 NS 和 NR 条件下，棕榈酸和油酸仍然是主要的脂肪酸，这表明 M. gracile 具有很高的生物柴油原料潜力。生物柴油特性分析显示，在两种处理方式下，十六烷值（CN 61.9-64.4）和不饱和度（DU 45.3-57.4）都很高。目前的研究结果表明，非模式微藻有可能继承优于模式物种的油脂积累能力，从而促进生物柴油的发展。
