Yapici Nazmiye B, Gao Xiang, Yan Xin, Hou Shanshan, Jockusch Steffen, Lesniak Lillian, Gibson K Michael, Bi Lanrong
Department of Chemistry, Michigan Technological University, Houghton, Michigan 49931, United States.
Department of Chemistry, Columbia University, New York, New York 10027, United States.
ACS Omega. 2021 Nov 16;6(47):31447-31456. doi: 10.1021/acsomega.1c03087. eCollection 2021 Nov 30.
Many organelles, such as lysosomes and mitochondria, maintain a pH that is different from the cytoplasmic pH. These pH differences have important functional ramifications for those organelles. Many cellular events depend upon a well-compartmentalized distribution of H ions spanning the membrane for the optimal function. Cells have developed a variety of mechanisms that enable the regulation of organelle pH. However, the measurement of organellar acidity/alkalinity in living cells has remained a challenge. Currently, most existing probes for the estimation of intracellular pH show a single -organelle targeting capacity. Such probes provide data that fails to comprehensively reveal the pathological and physiological roles and connections between mitochondria and lysosomes in different species. Mitochondrial and lysosomal functions are closely related and important for regulating cellular homeostasis. Accordingly, the design of a single fluorescent probe that can simultaneously target mitochondria and lysosomes is highly desirable, enabling a better understanding of the crosstalk between these organelles. We report the development of a novel fluorescent sensor, rhodamine-coumarin pH probe (RCPP), for detection of organellar acidity/alkalinity. RCPP simultaneously moves between mitochondrion and lysosome subcellular locations, facilitating the simultaneous monitoring of pH alterations in mitochondria and lysosomes.
许多细胞器，如溶酶体和线粒体，维持着与细胞质pH值不同的pH值。这些pH值差异对这些细胞器具有重要的功能影响。许多细胞活动依赖于跨膜H离子的良好区室化分布以实现最佳功能。细胞已经发展出多种机制来调节细胞器的pH值。然而，测量活细胞中细胞器的酸度/碱度仍然是一个挑战。目前，大多数现有的用于估计细胞内pH值的探针显示出单一细胞器靶向能力。此类探针提供的数据无法全面揭示不同物种中线粒体和溶酶体之间的病理和生理作用及联系。线粒体和溶酶体的功能密切相关，对调节细胞内稳态很重要。因此，非常需要设计一种能够同时靶向线粒体和溶酶体的单一荧光探针，以便更好地理解这些细胞器之间的相互作用。我们报告了一种新型荧光传感器罗丹明-香豆素pH探针（RCPP）的开发，用于检测细胞器的酸度/碱度。RCPP同时在线粒体和溶酶体亚细胞位置之间移动，便于同时监测线粒体和溶酶体中的pH值变化。
