Wetterwald A, Toffler O, de Weck A L, Blaser K
Mol Immunol. 1986 Mar;23(3):347-56. doi: 10.1016/0161-5890(86)90062-3.
Guinea pigs of strains 2 and 13 can produce isologous anti-idiotypic (aIds) antibodies against anti-benzylpenicilloyl (anti-BPO) IgG, following immunization with affinity-purified anti-BPO antibodies of the same strain. The specificity of aId was determined by inhibition of binding of aId to Fab(t) in ELISA. The results showed that the reaction of strain 2 (anti-BPO)aId can be inhibited with syngeneic anti-BPO Fab(t) and to a smaller degree with anti-BPO Fab(t) of strain 13. On the other hand, strain 13 (anti-BPO)aId reacted exclusively with syngeneic anti-BPO Fab(t). In both cases, binding of aId to anti-BPO Fab(t) could not be inhibited with BPO-epsilon-aminocaproic acid, indicating that these aId are not directed against the antigen-combining site. The effect of isologous aId on both short- and long-time established IgE responses was studied in guinea pigs of strain 13. In both situations, administration of isologous aId resulted in suppression of the anti-BPO IgE antibody response. The suppressive effect was antigen-specific and lasted for several weeks: in the case of an early-response IgE remained suppressed despite additional booster injections of antigen. In contrast to the IgE response, the production of anti-BPO IgG antibodies was only slightly affected.
2号和13号品系的豚鼠在用同品系的亲和纯化抗苄青霉素酰基（抗BPO）抗体免疫后，能够产生针对抗苄青霉素酰基（抗BPO）IgG的同种抗独特型（aIds）抗体。通过在酶联免疫吸附测定（ELISA）中抑制aId与Fab(t)的结合来确定aId的特异性。结果显示，2号品系（抗BPO）aId的反应可被同基因抗BPO Fab(t)抑制，被13号品系的抗BPO Fab(t)抑制的程度较小。另一方面，13号品系（抗BPO）aId仅与同基因抗BPO Fab(t)反应。在这两种情况下，BPO-ε-氨基己酸均不能抑制aId与抗BPO Fab(t)的结合，这表明这些aId并非针对抗原结合位点。在13号品系的豚鼠中研究了同种aId对短期和长期建立的IgE反应的影响。在这两种情况下，给予同种aId均导致抗BPO IgE抗体反应受到抑制。这种抑制作用具有抗原特异性且持续数周：在早期反应的情况下，尽管再次注射抗原进行加强免疫，IgE仍受到抑制。与IgE反应不同，抗BPO IgG抗体的产生仅受到轻微影响。
