Department of Biology, Department of Life and Nanopharmaceutical Sciences and Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005129.
Cultivation and isolation of gut bacteria are necessary for understanding their role in the intestinal ecosystem. We isolated a novel bacterium, designated strain BG01, from the faeces of a patient with Crohn's disease. Strain BG01 was a strictly anaerobic, rod-shaped, Gram-variable and endospore-forming bacterium. Strain BG01 possessed C, C dimethyl aldehyde (DMA) and C 9 DMA as predominant cellular fatty acids and -diaminopimelic acid as a diagnostic diamino acid. Strain BG01 grew at 15-45 °C (optimum, 37 °C), with 0-4 % (w/v) NaCl (optimum, 0-1 %), at pH 6-10 (optimum, pH 7) and was resistant to bile salt, but not to ampicillin, metronidazole, vancomycin and cefoperazone. Butyrate, propionate, oxalacetate and fumarate were produced as fermentation end products from Gifu anaerobic medium broth. Strain BG01 showed 97.7 % 16S rRNA gene sequence similarity, and 92.0 and 48.5 % of average nucleotide identity and digital DNA-DNA hybridization values, respectively, with KCTC 15019. Genomic analysis indicated that strain BG01 had a butyrate-producing pathway. The genomic G+C content of the strain was 43.5 mol%. Results of the phenotypic, phylogenetic and genotypic analyses indicated that strain BG01 represents a novel butyrate-producing species of the genus , for which the name sp. nov. is proposed. The type strain is BG01 (=KCTC 15617=JCM 32275).
肠道细菌的培养和分离对于了解其在肠道生态系统中的作用是必要的。我们从一名克罗恩病患者的粪便中分离到一株新型细菌，命名为 BG01 株。BG01 株是严格厌氧菌，呈杆状，革兰氏染色可变，形成芽孢。BG01 株的主要细胞脂肪酸为 C、C 二甲基乙醛（DMA）和 C9DMA，鉴别性二氨基庚二酸为 -二氨基庚二酸。BG01 株在 15-45°C（最佳温度为 37°C）、0-4%（w/v）NaCl（最佳浓度为 0-1%）、pH6-10（最佳 pH 值为 7）的条件下生长，能耐受胆盐，但不能耐受氨苄西林、甲硝唑、万古霉素和头孢哌酮。BG01 株从吉富厌氧液体培养基中发酵产生丁酸盐、丙酸盐、草酰乙酸盐和富马酸盐。BG01 株的 16S rRNA 基因序列相似度为 97.7%，平均核苷酸同一性和数字 DNA-DNA 杂交值分别与 KCTC 15019 株为 92.0%和 48.5%。基因组分析表明，BG01 株具有丁酸产生途径。该菌株的基因组 G+C 含量为 43.5mol%。表型、系统发育和基因型分析结果表明，BG01 株代表了一个新型丁酸产生菌，属于 属，提议将其命名为 sp. nov.。模式菌株为 BG01 株（=KCTC 15617=JCM 32275）。
