Dong Hanbo, Li Runzhe, Zhao Ni, Dadhania Darshana M, Suthanthiran Manikkam, Lee John R, Ling Wodan
Division of Biostatistics, Department of Population Health Sciences, Weill Cornell Medicine, New York, NY, United States.
Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.
Front Transplant. 2024 Sep 20;3:1400067. doi: 10.3389/frtra.2024.1400067. eCollection 2024.
The impact of antibiotics on the gut microbiota in kidney transplant recipients is not well characterized. In this study, we determine the impact of different subclasses of antibiotics on the gut microbiota in a cohort of 168 kidney transplant recipients.
Gut microbiome profiling was performed on 510 fecal specimens using 16S rRNA gene sequencing of the V4-V5 hypervariable region. We classified fecal specimens by antibiotic exposure into 5 categories: Beta-lactam, Fluoroquinolone (FQ), Beta-lactam & FQ Group, Other Antibiotics, and No Antibiotic (No Abx). Mixed-effects regression models were utilized to identify changes in microbial diversity and in the centered log-ratio (CLR) transformed abundance of genera while adjusting for important covariates.
Antibiotic administration was associated with a significant decrease in the Shannon alpha diversity index, a decreased abundance of 11 taxa including and , and an increased abundance of 16 taxa including and Exposure to Beta-lactam antibiotics was associated with an increased abundance of 10 taxa including and a decreased abundance of 5 taxa including while exposure to FQ antibiotics was associated with an increased abundance of 3 taxa and a decreased abundance of 4 taxa including .
Beta-lactam antibiotics and FQ antibiotics have a profound impact on the gut microbiota in kidney transplant recipients. Given the link of the gut microbiota to infectious complications, antibiotic associated changes in the microbiota may lead to an increased risk for further infections.
抗生素对肾移植受者肠道微生物群的影响尚未得到充分描述。在本研究中，我们确定了不同亚类抗生素对168名肾移植受者队列中肠道微生物群的影响。
使用V4-V5高变区的16S rRNA基因测序对510份粪便标本进行肠道微生物组分析。我们根据抗生素暴露情况将粪便标本分为5类：β-内酰胺类、氟喹诺酮类（FQ）、β-内酰胺类与FQ组、其他抗生素类和无抗生素（无抗生素）组。利用混合效应回归模型确定微生物多样性以及属的中心对数比（CLR）转换丰度的变化，同时对重要协变量进行调整。
抗生素给药与香农α多样性指数显著降低、11个分类群（包括 和 ）丰度降低以及16个分类群（包括 和 ）丰度增加有关。暴露于β-内酰胺类抗生素与10个分类群（包括 ）丰度增加和5个分类群（包括 ）丰度降低有关，而暴露于FQ抗生素与3个分类群丰度增加和4个分类群（包括 ）丰度降低有关。
β-内酰胺类抗生素和FQ抗生素对肾移植受者的肠道微生物群有深远影响。鉴于肠道微生物群与感染性并发症之间的联系，抗生素相关的微生物群变化可能会导致进一步感染的风险增加。
